Citation
Schneider Teixeira, Aline and Faltus, Milos and Zamecnik, Jiri and Kotková, Renata and González Benito, Elena and Molina García, Antonio Diego
(2012).
Invariance of the Glass Transition Temperature of Plant Vitrification Solutions with Cooling Rate.
"Cryobiology", v. 65
(n. 3);
p. 347.
ISSN 0011-2240.
Abstract
The solutions studied were Plant Vitrification Solutions 1, 2 and 3: (PVS1: Uragami et al. 1989, Plant Cell Rep. 8, 418; PVS2: Sakai et al. 1990, Plant Cell Rep. 9, 30; PVS3: Nishizawa et al. 1993, Plant Sci. 91, 67). Cooling was performed using the calorimeter control (5, 10 and 20°C min-1), or for higher rates, by quenching the closed pan with PVS in LN, either naked (faster - 5580°C min-1) or introduced in cryovials (reduced rate 360°C min-1). Quenched pans were then transferred to the sample chamber, pre-cooled to -196°C. Glass transition temperature was observed by DSC with a TA 2920 instrument, upon warming pans with solution samples from -145°C to room temperature, at standard warming rate10°C min-1.
