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Biomolecular interaction analysis of gestrinone-anti-gestrinone using arrays of high aspect ratio SU-8 nanopillars
Citation
Ortega, Francisco J., Bañuls, María José, Sanza Gutiérrez, Francisco Javier, Casquel del Campo, Rafael 
ORCID: https://orcid.org/0000-0003-2433-9159, Laguna Heras, Maria Fe ORCID: https://orcid.org/0000-0002-1580-4509, Holgado Bolaños, Miguel ORCID: https://orcid.org/0000-0001-9299-1371, López-Romero Moraleda, David, Angulo Barrios, Carlos ORCID: https://orcid.org/0000-0002-2496-5443, Maquieira, A. and Puchades, Rosa
(2012).
Biomolecular interaction analysis of gestrinone-anti-gestrinone using arrays of high aspect ratio SU-8 nanopillars.
"Biosensors", v. 2
(n. 3);
pp. 291-304.
ISSN 2079-6374.
https://doi.org/10.3390/bios2030291.
Description
| Title: | Biomolecular interaction analysis of gestrinone-anti-gestrinone using arrays of high aspect ratio SU-8 nanopillars |
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| Author/s: |
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| Item Type: | Article |
| Título de Revista/Publicación: | Biosensors |
| Date: | September 2012 |
| ISSN: | 2079-6374 |
| Volume: | 2 |
| Subjects: | |
| Freetext Keywords: | micro-nanofabrication; SU-8; optical interrogation; label-free nanobiosensing; gestrinone; immunoassay affinity constant determination |
| Faculty: | E.T.S.I. Telecomunicación (UPM) |
| Department: | Ingeniería Electrónica |
| Creative Commons Licenses: | Recognition - No derivative works - Non commercial |
Abstract
In this paper, label-free biosensing for antibody screening by periodic lattices of high-aspect ratio SU-8 nano-pillars (BICELLs) is presented. As a demonstration, the determination of anti-gestrinone antibodies from whole rabbit serum is carried out, and for the first time, the dissociation constant (KD = 6 nM) of antigen-antibody recognition process is calculated using this sensing system. After gestrinone antigen immobilization on the BICELLs, the immunorecognition was performed. The cells were interrogated vertically by using micron spot size Fourier transform visible and IR spectrometry (FT-VIS-IR), and the dip wavenumber shift was monitored. The biosensing assay exhibited good reproducibility and sensitivity (LOD = 0.75 ng/mL).
More information
| Item ID: | 16735 |
|---|---|
| DC Identifier: | https://oa.upm.es/16735/ |
| OAI Identifier: | oai:oa.upm.es:16735 |
| DOI: | 10.3390/bios2030291 |
| Official URL: | http://www.mdpi.com/2079-6374/2/3/291 |
| Deposited by: | Memoria Investigacion |
| Deposited on: | 07 Aug 2013 15:42 |
| Last Modified: | 28 Jun 2016 07:01 |
