Functional roles of HypC and HupK in the biosynthesis of [NiFe] hydrogenase in Rhizobium leguminosarum.

Albareda Contreras, Marta ORCID: https://orcid.org/0000-0002-4343-4445, Manyani, Hamid, Rey Navarro, Luis ORCID: https://orcid.org/0000-0003-3477-6942, Brito Lopez, Maria Belen, Ruiz Argüeso, Tomas-Andres, Imperial Ródenas, Juan ORCID: https://orcid.org/0000-0002-5002-6458, Pacios, Luis F. and Palacios Alberti, Jose Manuel ORCID: https://orcid.org/0000-0002-2541-8812 (2013). Functional roles of HypC and HupK in the biosynthesis of [NiFe] hydrogenase in Rhizobium leguminosarum.. In: "II IBEMPA. Conference. Microorganisms for future Agriculture", 02/09/2013 - 06/09/2013, Sevilla. ISBN 978-84-616-6794-9. p. 364.

Description

Title: Functional roles of HypC and HupK in the biosynthesis of [NiFe] hydrogenase in Rhizobium leguminosarum.
Author/s:
Item Type: Presentation at Congress or Conference (Article)
Event Title: II IBEMPA. Conference. Microorganisms for future Agriculture
Event Dates: 02/09/2013 - 06/09/2013
Event Location: Sevilla
Title of Book: II IBEMPA Conference. Microorganisms for future Agriculture
Date: 2013
ISBN: 978-84-616-6794-9
Subjects:
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Some rhizobia induce a hydrogen (H2)-uptake system with a [NiFe] hydrogenase along with nitrogenase to recover part of the energy lost as H2. Biosynthesis of NiFe hydrogenases is a process that ocurrs in the cytoplasm, where a number of auxiliary proteins (products of hup and hyp genes) are required to synthesize and insert the metal cofactors into the enzyme structural units. Although HypC is expressed in all hydrogenase systems, HupF and HupK are found only in bacteria that express the hydrogenase in the presence of oxygen (O2). Co-purification experiments have demonstrated HypC-HupK and HypC-HupL interactions. Results have shown that some conserved residues from HypC and HupK play a protective role of hydrogenase against the presence of O2.

More information

Item ID: 29865
DC Identifier: https://oa.upm.es/29865/
OAI Identifier: oai:oa.upm.es:29865
Official URL: http://congreso.us.es/ibempa/images/Abstracts-book...
Deposited by: Memoria Investigacion
Deposited on: 30 Oct 2014 17:52
Last Modified: 30 Oct 2014 17:52
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