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Zarouri, Belkacem, Vargas, Alba María, Gaforio, Laura, Aller, María, Andrés, María Teresa de and Cabezas, José Antonio (2015). Whole-genome genotyping of grape using a panel of microsatellite. "Tree Genetics & Genomes" ; pp. 17-32. ISSN 1614-2942. https://doi.org/10.1007/s11295-015-0843-4.
Title: | Whole-genome genotyping of grape using a panel of microsatellite |
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Author/s: |
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Item Type: | Article |
Título de Revista/Publicación: | Tree Genetics & Genomes |
Date: | February 2015 |
ISSN: | 1614-2942 |
Subjects: | |
Faculty: | E.T.S.I. Agrónomos (UPM) [antigua denominación] |
Department: | Otro |
Creative Commons Licenses: | Recognition - No derivative works - Non commercial |
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The use of microsatellite markers in large-scale genetic studies is limited by its low throughput and high cost and labor requirements. Here, we provide a panel of 45 multiplex PCRs for fast and cost-efficient genome-wide fluorescence-based microsatellite analysis in grapevine. The developed multiplex PCRs panel (with up to 15-plex) enables the scoring of 270 loci covering all the grapevine genome (9 to 20 loci/chromosome) using only 45 PCRs and sequencer runs. The 45 multiplex PCRs were validated using a diverse grapevine collection of 207 accessions, selected to represent most of the cultivated Vitis vinifera genetic diversity. Particular attention was paid to quality control throughout the whole process (assay replication, null allele detection, ease of scoring). Genetic diversity summary statistics and features of electrophoretic profiles for each studied marker are provided, as are the genotypes of 25 common cultivars that could be used as references in other studies.
Item ID: | 37978 |
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DC Identifier: | https://oa.upm.es/37978/ |
OAI Identifier: | oai:oa.upm.es:37978 |
DOI: | 10.1007/s11295-015-0843-4 |
Official URL: | http://link.springer.com/article/10.1007/s11295-01... |
Deposited by: | Memoria Investigacion |
Deposited on: | 05 Nov 2015 13:50 |
Last Modified: | 03 Jun 2019 13:59 |