Full text
Preview |
PDF
- Requires a PDF viewer, such as GSview, Xpdf or Adobe Acrobat Reader
Download (193kB) | Preview |
Optimization of Dengue immunoassay by label-free interferometric optical detection method
Citation
Laguna Heras, Maria Fe 
ORCID: https://orcid.org/0000-0002-1580-4509, Holgado Bolaños, Miguel ORCID: https://orcid.org/0000-0001-9299-1371, Sanza Gutiérrez, Francisco Javier, Lavin Hueros, Alvaro ORCID: https://orcid.org/0000-0001-9981-2372, López Hernández, Ana ORCID: https://orcid.org/0000-0002-4415-1826 and Casquel del Campo, Rafael ORCID: https://orcid.org/0000-0003-2433-9159
(2014).
Optimization of Dengue immunoassay by label-free interferometric optical detection method.
"Sensors", v. 14
;
pp. 6695-6700.
ISSN 1424-8220.
https://doi.org/10.3390/s140406695.
Description
| Title: | Optimization of Dengue immunoassay by label-free interferometric optical detection method |
|---|---|
| Author/s: |
|
| Item Type: | Article |
| Título de Revista/Publicación: | Sensors |
| Date: | April 2014 |
| ISSN: | 1424-8220 |
| Volume: | 14 |
| Subjects: | |
| Freetext Keywords: | photonic sensing cells; biosensors; label-free immunoassay; dengue virus |
| Faculty: | E.T.S.I. Industriales (UPM) |
| Department: | Física Aplicada e Ingeniería de Materiales |
| UPM's Research Group: | Group of Micro-nano Photonics and Biophotonics, Laser Center, and Center for Biomedical Technology |
| Creative Commons Licenses: | Recognition - No derivative works - Non commercial |
Abstract
In this communication we report a direct immunoassay for detecting dengue virus by means of a label-free interferometric optical detection method. We also demonstrate how we can optimize this sensing response by adding a blocking step able to significantly enhance the optical sensing response. The blocking reagent used for this optimization is a dry milk diluted in phosphate buffered saline. The recognition curve of dengue virus over the proposed surface sensor demonstrates the capacity of this method to be applied in Point of Care technology.
Funding Projects
Type
Code
Acronym
Leader
Title
Government of Spain
IPT-2011-1429-010000
Unspecified
Unspecified
Project INNBIOD
Government of Spain
TEC20121-31145
Unspecified
Unspecified
Project PLATON
FP7
FP7 - OCEAN - 2013
Unspecified
Unspecified
ENVIGUARD EUROPEAN PROJECT
More information
| Item ID: | 40118 |
|---|---|
| DC Identifier: | https://oa.upm.es/40118/ |
| OAI Identifier: | oai:oa.upm.es:40118 |
| DOI: | 10.3390/s140406695 |
| Official URL: | http://www.mdpi.com/journal/sensors |
| Deposited by: | Memoria Investigacion |
| Deposited on: | 05 May 2016 15:21 |
| Last Modified: | 05 May 2016 15:21 |
