Citation
Cañón Beltrán, K. and Cajas, Y.N. and García García, R. M. and Lorenzo, P.L. and Carrera, R. and Garcia Rebollar, Pilar and Arias Álvarez, María
(2015).
Effect of epidermal growth factor on nuclear and cytoplasmic in vitro maruration of guinea pig oocytes.
"Animal Reproduction", v. 12
(n. 3);
p. 793.
ISSN 1806-9614.
Abstract
The guinea pig may represent an animal model for research on ovarian infertility and improvement of the in vitro
maturation (IVM) conditions is needed in this species. The aim of the present work was to immunolocalize the Epidermal Growth Factor (EGF)-Receptor in the guinea pig ovaries and to study the effect of EGF on meiotic and cytoplasmic maturation, and apoptotic rate in cumulus-oocyte-co
mplexes (COCs). Immunohistochemistry was performed in paraffined ovaries using a rabbit polyclonal antibody EGF-R (1:100; Santa Cruz Biotechnology) and the ABC Vector Elite kit (Vector Laboratories). For the IVM, COCs were collected by aspiration of follicles >700μm under a stereoscopic microscope.
