Topographical and mechanical characterization of living eukaryotic cells on opaque substrates: development of a general procedure and its application to the study of non-adherent lymphocytes

Daza García, Rafael ORCID: https://orcid.org/0000-0001-7883-648X, Cruces, Julia, Arroyo-Hernández, María, Marí-Buyé, Núria, Fernández de la Fuente, Mónica, Plaza Baonza, Gustavo Ramón ORCID: https://orcid.org/0000-0002-5555-5498, Elices Calafat, Manuel, Pérez Rigueiro, José ORCID: https://orcid.org/0000-0001-8298-8398 and Guinea Tortuero, Gustavo V. ORCID: https://orcid.org/0000-0002-4326-3746 (2015). Topographical and mechanical characterization of living eukaryotic cells on opaque substrates: development of a general procedure and its application to the study of non-adherent lymphocytes. "Physical Biology", v. 12 (n. 2); pp. 1-12. ISSN 1478-3967. https://doi.org/10.1088/1478-3975/12/2/026005.

Description

Title: Topographical and mechanical characterization of living eukaryotic cells on opaque substrates: development of a general procedure and its application to the study of non-adherent lymphocytes
Author/s:
Item Type: Article
Título de Revista/Publicación: Physical Biology
Date: 19 March 2015
ISSN: 1478-3967
Volume: 12
Subjects:
Freetext Keywords: Atomic force microscopy; cell; mechanics
Faculty: E.T.S.I. Caminos, Canales y Puertos (UPM)
Department: Ciencia de los Materiales
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

The mechanical behavior of living murine T-lymphocytes was assessed by atomic force microscopy (AFM). A robust experimental procedure was developed to overcome some features of lymphocytes, in particular their spherical shape and non-adherent character. The procedure included the immobilization of the lymphocytes on amine-functionalized substrates, the use of hydrodynamic effects on the deflection of the AFM cantilever to monitor the approaching, and the use of the jumping mode for obtaining the images. Indentation curves were analyzed according to Hertz's model for contact mechanics. The calculated values of the elastic modulus are consistent both when considering the results obtained from a single lymphocyte and when comparing the curves recorded from cells of different specimens

Funding Projects

Type
Code
Acronym
Leader
Title
Government of Spain
BFU2011-30336
Unspecified
Unspecified
Unspecified
Madrid Regional Government
S2011/BMD-2460
Unspecified
Unspecified
Unspecified
Government of Spain
MAT2009-10258
Unspecified
Unspecified
Unspecified

More information

Item ID: 41137
DC Identifier: https://oa.upm.es/41137/
OAI Identifier: oai:oa.upm.es:41137
DOI: 10.1088/1478-3975/12/2/026005
Official URL: http://iopscience.iop.org/article/10.1088/1478-397...
Deposited by: Memoria Investigacion
Deposited on: 29 Jun 2016 14:14
Last Modified: 30 Nov 2022 09:00
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