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Daza García, Rafael ORCID: https://orcid.org/0000-0001-7883-648X, Cruces, Julia, Arroyo-Hernández, María, Marí-Buyé, Núria, Fernández de la Fuente, Mónica, Plaza Baonza, Gustavo Ramón
ORCID: https://orcid.org/0000-0002-5555-5498, Elices Calafat, Manuel, Pérez Rigueiro, José
ORCID: https://orcid.org/0000-0001-8298-8398 and Guinea Tortuero, Gustavo V.
ORCID: https://orcid.org/0000-0002-4326-3746
(2015).
Topographical and mechanical characterization of living eukaryotic cells on opaque substrates: development of a general procedure and its application to the study of non-adherent lymphocytes.
"Physical Biology", v. 12
(n. 2);
pp. 1-12.
ISSN 1478-3967.
https://doi.org/10.1088/1478-3975/12/2/026005.
Title: | Topographical and mechanical characterization of living eukaryotic cells on opaque substrates: development of a general procedure and its application to the study of non-adherent lymphocytes |
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Author/s: |
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Item Type: | Article |
Título de Revista/Publicación: | Physical Biology |
Date: | 19 March 2015 |
ISSN: | 1478-3967 |
Volume: | 12 |
Subjects: | |
Freetext Keywords: | Atomic force microscopy; cell; mechanics |
Faculty: | E.T.S.I. Caminos, Canales y Puertos (UPM) |
Department: | Ciencia de los Materiales |
Creative Commons Licenses: | Recognition - No derivative works - Non commercial |
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The mechanical behavior of living murine T-lymphocytes was assessed by atomic force microscopy (AFM). A robust experimental procedure was developed to overcome some features of lymphocytes, in particular their spherical shape and non-adherent character. The procedure included the immobilization of the lymphocytes on amine-functionalized substrates, the use of hydrodynamic effects on the deflection of the AFM cantilever to monitor the approaching, and the use of the jumping mode for obtaining the images. Indentation curves were analyzed according to Hertz's model for contact mechanics. The calculated values of the elastic modulus are consistent both when considering the results obtained from a single lymphocyte and when comparing the curves recorded from cells of different specimens
Item ID: | 41137 |
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DC Identifier: | https://oa.upm.es/41137/ |
OAI Identifier: | oai:oa.upm.es:41137 |
DOI: | 10.1088/1478-3975/12/2/026005 |
Official URL: | http://iopscience.iop.org/article/10.1088/1478-397... |
Deposited by: | Memoria Investigacion |
Deposited on: | 29 Jun 2016 14:14 |
Last Modified: | 30 Nov 2022 09:00 |