Monitoring of airborne biological particles in outdoor atmosphere. Part 2: Metagenomics applied to urban environments

Núñez, Andrés, Amo de Paz, Guillermo, Rastrojo, Alberto, García Ruiz, Ana María ORCID: https://orcid.org/0000-0002-2210-3778, Alcamí, Antonio, Gutiérrez-Bustillo, A. Montserrat and Moreno Gómez, Diego Alejandro ORCID: https://orcid.org/0000-0003-2271-7013 (2016). Monitoring of airborne biological particles in outdoor atmosphere. Part 2: Metagenomics applied to urban environments. "International Microbiology", v. 19 ; pp. 69-80. ISSN 1139-6709 e-ISSN: 1618-1095. https://doi.org/10.2436/20.1501.01.265.

Description

Title: Monitoring of airborne biological particles in outdoor atmosphere. Part 2: Metagenomics applied to urban environments
Author/s:
Item Type: Article
Título de Revista/Publicación: International Microbiology
Date: April 2016
ISSN: 1139-6709 e-ISSN: 1618-1095
Volume: 19
Subjects:
Freetext Keywords: airborne biological particles, metagenomics, next-generation sequencing (NGS), air biomonitoring, urban aerobiology
Faculty: E.T.S.I. Industriales (UPM)
Department: Física Aplicada e Ingeniería de Materiales
Creative Commons Licenses: Recognition - No derivative works - Non commercial

Full text

[thumbnail of INVE_MEM_2016_250891.pdf]
Preview
PDF - Requires a PDF viewer, such as GSview, Xpdf or Adobe Acrobat Reader
Download (1MB) | Preview

Abstract

The air we breathe contains microscopic biological particles such as viruses, bacteria, fungi and pollen, some of them with relevant clinic importance. These organisms and/or their propagules have been traditionally studied by different disciplines and diverse methodologies like culture and microscopy. These techniques require time, expertise and also have some important biases. As a consequence, our knowledge on the total diversity and the relationships between the different biological entities present in the air is far from being complete. Currently, metagenomics and next-generation sequencing (NGS) may resolve this shortage of information and have been recently applied to metropolitan areas. Although the procedures and methods are not totally standardized yet, the first studies from urban air samples confirm the previous results obtained by culture and microscopy regarding abundance and variation of these biological particles. However, DNA-sequence analyses call into question some preceding ideas and also provide new interesting insights into diversity and their spatial distribution inside the cities. Here, we review the procedures, results and perspectives of the recent works that apply NGS to study the main biological particles present in the air of urban environments.

Funding Projects

Type
Code
Acronym
Leader
Title
Madrid Regional Government
S2013/MAE-2874
AIRBIOTA
Diego Alejandro Moreno Gómez
Conocer y modelizar la contaminación biológica del aire urbano

More information

Item ID: 45939
DC Identifier: https://oa.upm.es/45939/
OAI Identifier: oai:oa.upm.es:45939
DOI: 10.2436/20.1501.01.265
Official URL: http://revistes.iec.cat/index.php/IM/article/viewF...
Deposited by: Memoria Investigacion
Deposited on: 19 May 2017 09:34
Last Modified: 30 Nov 2022 09:00
  • Logo InvestigaM (UPM)
  • Logo GEOUP4
  • Logo Open Access
  • Open Access
  • Logo Sherpa/Romeo
    Check whether the anglo-saxon journal in which you have published an article allows you to also publish it under open access.
  • Logo Dulcinea
    Check whether the spanish journal in which you have published an article allows you to also publish it under open access.
  • Logo de Recolecta
  • Logo del Observatorio I+D+i UPM
  • Logo de OpenCourseWare UPM