Citation
Cubells Baeza, Nuria and Gomez Casado, Cristina and Tordesillas Villuendas, Leticia and Ramírez Castillejo, Carmen and Garrido Arandia, M. and González Melendi, Pablo and Herrero, María and Pacios, Luis F. and Díaz Perales, Araceli
(2017).
Identification of the ligand of Pru p 3, a peach LTP.
"Plant Molecular Biology", v. 94
(n. 1-2);
pp. 33-44.
ISSN 0167-4412.
https://doi.org/10.1007/s11103-017-0590-z.
Abstract
The allergen Pru p 3, a peach lipid transfer protein, has been well studied. However, its physiological function remains to be elucidated. Our results showed that Pru p 3 usually carries a lipid ligand that play an essential role in its function in plants. Using ESI-qToF, we observed that the ligand was a derivative of camptothecin binding to phytosphingosine, wich that is inserted into the hydrophobic tunnel of the protein. In addition, the described ligand displayed topoisomerase I activity inhibition and self-fluorescence, both recognized as camptothecin properties. During flower development, the highest expression of Pru p 3 was detected in the styles of pollinated flowers, in contrast to its non-expression in unpollinated pistils, where expression decreased after anthesis. During ripening, the expression of Pru p 3 were observed mainly in peel but not in pulp. In this sense, Pru p 3 protein was also localized in trichomes covering the fruit epidermis.
