Component-Resolved in Vitro Diagnosis in Peach-Allergic Patients

Gamboa, P.M., Sanz, M.L., Lombardero, M., Barber, Domingo, Sánchez-Monge Laguna de Rins, Rosa, Goikoetxea, M.J., Antepara, I., Ferrer, M. and Salcedo Duran, Gabriel (2009). Component-Resolved in Vitro Diagnosis in Peach-Allergic Patients. "Journal of Investigational Allergology and Clinical Immunology", v. 19 (n. 1); pp. 13-20. ISSN 1018-9068.


Title: Component-Resolved in Vitro Diagnosis in Peach-Allergic Patients
  • Gamboa, P.M.
  • Sanz, M.L.
  • Lombardero, M.
  • Barber, Domingo
  • Sánchez-Monge Laguna de Rins, Rosa
  • Goikoetxea, M.J.
  • Antepara, I.
  • Ferrer, M.
  • Salcedo Duran, Gabriel
Item Type: Article
Título de Revista/Publicación: Journal of Investigational Allergology and Clinical Immunology
Date: January 2009
ISSN: 1018-9068
Volume: 19
Freetext Keywords: Peach allergy. Component-resolved in vitro diagnosis. Basophil Activation Test. Histamine Release Test. Sulfi doleukotriene determination
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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BACKGROUND: The in vitro diagnosis of pollen-related food allergy presents low specifi city and reproducibility with many conventional extracts. This can be improved using natural purifi ed allergens, recombinant purifi ed allergens, or both. OBJECTIVE: We compared specifi c immunoglobulin (Ig) E determination (sIgE), the basophil activation test (BAT), the histamine release test (HRT), and the cellular allergen stimulation test (CAST) using natural and recombinant allergens in the diagnosis of peach allergy. METHODS: Thirty-two peach allergic patients were studied. Skin prick tests were performed with commercial peach and extract with Mal d 1, nPru p 3, and profi lin (nPho d 2). sIgE, BAT, CAST, and HRT were determined using rPru p 3, rMal d 3, rBet v 1, rMal d 1, and rMal d 4. RESULTS: Agreement between the techniques was good with all the allergens, except HRT with rMal d 1 and rMal d 4. With rPru p 3, sIgE, CAST, BAT, and HRT showed sensitivity values of 88%, 81%, 72%, and 69% and specifi city values of 100%, 93%, 97%, and 83%, respectively. In patients with systemic symptoms or contact urticaria, the values were 100%, 85%, 81%, and 81%. In patients with oral allergy syndrome, sensitivity to profi lins or homologues of Bet v 1 was detected in 100% of the cases by all the techniques, except by HRT with rMal d 1, which detected 66% of the cases. CONCLUSIONS: The use of single allergens in the in vitro diagnosis of peach allergy by specifi c IgE determination, BAT, and CAST offers high specifi city and sensitivity, with better results than the HRT.

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Item ID: 5060
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Deposited by: Memoria Investigacion
Deposited on: 25 Nov 2010 10:23
Last Modified: 20 Apr 2016 14:01
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