Citation
Paz Ares, Javier and Valencia, Alfonso and Costantino, Paolo and Vittorioso, Paola and Davies, Brendan and Gilmartin, Phil and Giraudat, Jerome and Parcy, François and Reindl, Andreas and Sablowski, Robert and Coupland, George and Martin, Cathie and Angenent, Cerco C. and Baumlein, Helmut and Mock, Hans Peter and Carbonero Zalduegui, Pilar and Colombo, Lucia and Tonelli, Chiara and Engström, Peter and Dröege-Laser, Wolfgang and Gatz, Christiane and Kavanagh, Tony and Kushnir, Sergei and Zabeau, Marc and Laux, Thomas and Hordsworth, Mike and Ruberti, Ida and Ratcliff, Frank and Smeekens, Sjef and Somssich, Imre and Weisshaar, Bernd and Traas, Jan
(2002).
REGIA, an EU project on functional genomics of transcription factors from Arabidopsis thaliana.
"Comparative and Functional Genomics", v. 3
(n. 2);
pp. 102-108.
ISSN 1531-6912.
https://doi.org/10.1002/cfg.146.
Abstract
Transcription factors (TFs) are regulatory proteins that have played a pivotal role in the
evolution of eukaryotes and that also have great biotechnological potential. REGIA
(REgulatory Gene Initiative in Arabidopsis) is an EU-funded project involving 29
European laboratories with the objective of determining the function of virtually all
transcription factors from the model plant, Arabidopsis thaliana. REGIA involves: 1. the
definition of TF gene expression patterns in Arabidopsis; 2. the identification of mutations
at TF loci; 3. the ectopic expression of TFs (or derivatives) in Arabidopsis and in crop
plants; 4. phenotypic analysis of the mutants and mis-expression lines, including both RNA
and metabolic profiling; 5. the systematic analysis of interactions between TFs; and 6. the
generation of a bioinformatics infrastructure to access and integrate all this information.
We expect that this programme will establish the full biotechnological potential of plant
TFs, and provide insights into hierarchies, redundancies, and interdependencies, and their
evolution. The project involves the preparation of both a TF gene array for expression
analysis and a normalised full length open reading frame (ORF) library of TFs in a yeast
two hybrid vector; the applications of these resources should extend beyond the scope of
this programme