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Alfonso Rubí, Julio and Carbonero Zalduegui, Pilar and Diaz Rodriguez, Isabel (1999). Parameters influencing the regeneration capacity of calluses derived from mature indica and japonica rice seeds after microprojectile bombardment. "Euphytica", v. 107 (n. 2); pp. 115-122. ISSN 0014-2336. https://doi.org/10.1023/A:1026434228146.
Title: | Parameters influencing the regeneration capacity of calluses derived from mature indica and japonica rice seeds after microprojectile bombardment |
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Author/s: |
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Item Type: | Article |
Título de Revista/Publicación: | Euphytica |
Date: | June 1999 |
ISSN: | 0014-2336 |
Volume: | 107 |
Subjects: | |
Faculty: | E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM) |
Department: | Biotecnología - Biología Vegetal |
Creative Commons Licenses: | Recognition - No derivative works - Non commercial |
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The microprojectile bombardment procedure has allowed the stable transformation of indica and japonica rice varieties, although at different frequencies of transformation depending mainly on their regeneration capacity and on the specific parameters of the transformation protocol. A study of the process of regeneration to whole plants from primary calli derived from mature indica and japonica rice seeds, via embryogenesis, has shown that somatic embryos are produced by division and differentiation of the external cell layers of callus tissues. Adjusting the bombardment conditions to optimize gene delivery to those regenerable cells, we have evaluated the influence of parameters such as the target distance, particle penetration and the effect of osmotic treatment on the regeneration capacity of bombarded cells.
Type | Code | Acronym | Leader | Title |
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Government of Spain | BIO96-2303 | Unspecified | Pilar Carbonero | Identificación y mutagenización de genes de cereales para su utilización transgénica frente a plagas de insectos |
Government of Spain | BIO95-2061-E | Unspecified | Pilar Carbonero | Arroz transgénico resistente a plagas de insectos |
Item ID: | 53943 |
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DC Identifier: | https://oa.upm.es/53943/ |
OAI Identifier: | oai:oa.upm.es:53943 |
DOI: | 10.1023/A:1026434228146 |
Official URL: | https://link.springer.com/article/10.1023/A:1026434228146 |
Deposited by: | Biblioteca ETSI Agrónomos |
Deposited on: | 12 Feb 2019 09:37 |
Last Modified: | 12 Feb 2019 09:37 |