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Altpeter, Fredy, Diaz Rodriguez, Isabel ORCID: https://orcid.org/0000-0001-9865-902X, McAuslane, Heather, Gaddour, Kamel, Carbonero Zalduegui, Pilar
ORCID: https://orcid.org/0000-0002-4949-3577 and Vasil, Indra K.
(1999).
Increased insect resistance in transgenic wheat stably expressing trypsin inhibitor CMe.
"Molecular Breeding", v. 5
(n. 1);
pp. 56-63.
ISSN 1380-3743.
https://doi.org/10.1023/A:10096599.
Title: | Increased insect resistance in transgenic wheat stably expressing trypsin inhibitor CMe |
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Author/s: |
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Item Type: | Article |
Título de Revista/Publicación: | Molecular Breeding |
Date: | January 1999 |
ISSN: | 1380-3743 |
Volume: | 5 |
Subjects: | |
Faculty: | E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM) |
Department: | Biotecnología - Biología Vegetal |
Creative Commons Licenses: | Recognition - No derivative works - Non commercial |
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Proteinase inhibitors have been proposed to function as plant defence agents against herbivorous pests. We have introduced the barley trypsin inhibitor CMe (BTI-CMe) into wheat (Triticum aestivum L.) by biolistic bombardment of cultured immature embryos. Of the 30 independent transgenic wheat lines selected, 16 expressed BTI-CMe. BTI-CMe was properly transcribed and translated as indicated by northern and western blot, with a level of expression in transgenic wheat seeds up to 1.1% of total extracted protein. No expression was detected in untransformed wheat seeds. Functional integrity of BTI-CMe was confirmed by trypsin inhibitor activity assay. The significant reduction of the survival rate of the Angoumois grain moth (Sitotroga cerealella, Lepidoptera: Gelechiidae), reared on transgenic wheat seeds expressing the trypsin inhibitor BTI-CMe, compared to the untransformed control confirmed the potential of BTI-CMe for the increase of insect resistance. However, only early-instar larvae were inhibited in transgenic seeds and expression of BTI-CMe protein in transgenic leaves did not have a significant protective effect against leaf-feeding insects.
Item ID: | 53944 |
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DC Identifier: | https://oa.upm.es/53944/ |
OAI Identifier: | oai:oa.upm.es:53944 |
DOI: | 10.1023/A:10096599 |
Official URL: | https://link.springer.com/article/10.1023/A:100965... |
Deposited by: | Biblioteca ETSI Agronómica, Alimentaria y de Biosistemas |
Deposited on: | 12 Feb 2019 07:00 |
Last Modified: | 12 Feb 2019 07:42 |