Increased insect resistance in transgenic wheat stably expressing trypsin inhibitor CMe

Altpeter, Fredy, Diaz Rodriguez, Isabel ORCID: https://orcid.org/0000-0001-9865-902X, McAuslane, Heather, Gaddour, Kamel, Carbonero Zalduegui, Pilar ORCID: https://orcid.org/0000-0002-4949-3577 and Vasil, Indra K. (1999). Increased insect resistance in transgenic wheat stably expressing trypsin inhibitor CMe. "Molecular Breeding", v. 5 (n. 1); pp. 56-63. ISSN 1380-3743. https://doi.org/10.1023/A:10096599.

Description

Title: Increased insect resistance in transgenic wheat stably expressing trypsin inhibitor CMe
Author/s:
Item Type: Article
Título de Revista/Publicación: Molecular Breeding
Date: January 1999
ISSN: 1380-3743
Volume: 5
Subjects:
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Proteinase inhibitors have been proposed to function as plant defence agents against herbivorous pests. We have introduced the barley trypsin inhibitor CMe (BTI-CMe) into wheat (Triticum aestivum L.) by biolistic bombardment of cultured immature embryos. Of the 30 independent transgenic wheat lines selected, 16 expressed BTI-CMe. BTI-CMe was properly transcribed and translated as indicated by northern and western blot, with a level of expression in transgenic wheat seeds up to 1.1% of total extracted protein. No expression was detected in untransformed wheat seeds. Functional integrity of BTI-CMe was confirmed by trypsin inhibitor activity assay. The significant reduction of the survival rate of the Angoumois grain moth (Sitotroga cerealella, Lepidoptera: Gelechiidae), reared on transgenic wheat seeds expressing the trypsin inhibitor BTI-CMe, compared to the untransformed control confirmed the potential of BTI-CMe for the increase of insect resistance. However, only early-instar larvae were inhibited in transgenic seeds and expression of BTI-CMe protein in transgenic leaves did not have a significant protective effect against leaf-feeding insects.

Funding Projects

Type
Code
Acronym
Leader
Title
Government of Spain
BIO96-2303
Unspecified
Pilar Carbonero Zalduegui
Identificación y mutagenización de genes de cereales para su utilización transgénica frente a plagas de insectos

More information

Item ID: 53944
DC Identifier: https://oa.upm.es/53944/
OAI Identifier: oai:oa.upm.es:53944
DOI: 10.1023/A:10096599
Official URL: https://link.springer.com/article/10.1023/A:100965...
Deposited by: Biblioteca ETSI Agronómica, Alimentaria y de Biosistemas
Deposited on: 12 Feb 2019 07:00
Last Modified: 12 Feb 2019 07:42
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