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Gene specificity is maintained in transient expression assays with protoplasts derived from different tissues of barley
Citation
Diaz Rodriguez, Isabel and Royo, Joaquin and Sanchez de la Hoz, Pilar and Carbonero Zalduegui, Pilar (1995). Gene specificity is maintained in transient expression assays with protoplasts derived from different tissues of barley. "Euphytica", v. 85 (n. 1-3); pp. 203-207. ISSN 0014-2336. https://doi.org/10.1007/BF00023949.
Description
| Title: | Gene specificity is maintained in transient expression assays with protoplasts derived from different tissues of barley |
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| Author/s: |
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| Item Type: | Article |
| Título de Revista/Publicación: | Euphytica |
| Date: | February 1995 |
| ISSN: | 0014-2336 |
| Volume: | 85 |
| Subjects: | |
| Faculty: | E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM) |
| Department: | Biotecnología - Biología Vegetal |
| Creative Commons Licenses: | Recognition - No derivative works - Non commercial |
Abstract
In sorne cereal species that are still recalcitrant to stable transformation and regeneration, transient expression in isolated protoplasts is a useful tool for the study of gene expression and regulation. We have successfully applied these techniques to barley protoplasts derived from developing endosperm, aleurone, leaves and roots in order to characterize functionally cis-acting motives in two gene promoters, corresponding to trypsin inhibitor BTI-CMe and to sucrose synthase Ss1. Gene specificity is maintained in transient expression assays with protoplasts isolated from these different barley tissues and the pattem of expression parallels the mRNA levels observed for the corresponding genes in the same tissues.
Funding Projects
| Type | Code | Acronym | Leader | Title |
|---|---|---|---|---|
| Government of Spain | BIO93-1184 | Unspecified | Unspecified | Unspecified |
| Government of Spain | BIO91-0782 | Unspecified | Pilar Carbonero | Caracterización molecular de una familia de inhibidores de proteasas/alfa-amilasas de cereales y utilización de los clones correspondientes para producir plantas transgénicas resistentes a insectos fitófagos |
More information
| Item ID: | 53987 |
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| DC Identifier: | https://oa.upm.es/53987/ |
| OAI Identifier: | oai:oa.upm.es:53987 |
| DOI: | 10.1007/BF00023949 |
| Official URL: | https://link.springer.com/article/10.1007/BF00023949 |
| Deposited by: | Biblioteca ETSI Agrónomos |
| Deposited on: | 14 Feb 2019 08:38 |
| Last Modified: | 14 Feb 2019 08:38 |
