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Diaz Rodriguez, Isabel and Carbonero Zalduegui, Pilar (1992). Isolation of Protoplasts from Developing Barley Endosperm: a Tool for Transient Expression Studies. "Plant Cell Reports", v. 10 (n. 12); pp. 595-598. ISSN 0721-7714. https://doi.org/10.1007/BF00232377.
Title: | Isolation of Protoplasts from Developing Barley Endosperm: a Tool for Transient Expression Studies |
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Author/s: |
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Item Type: | Article |
Título de Revista/Publicación: | Plant Cell Reports |
Date: | January 1992 |
ISSN: | 0721-7714 |
Volume: | 10 |
Subjects: | |
Faculty: | E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM) |
Department: | Biotecnología - Biología Vegetal |
Creative Commons Licenses: | Recognition - No derivative works - Non commercial |
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We have developed a method for the routine isolation of protoplasts from developing starchy endosperm of barley (Hordeum vulgare L.). Preplasmolysis of the intact endosperms, a low concentration of hydrolytic enzymes and gravity sedimentation before any centrifugation step, were crucial factors for a good preparation. Best yields were obtained early after pollination (8–13 days) or with mutants with low starch content. Transient expression of a reporter gene under the control of the 35S promoter, after polyethyleneglycol transfection of endosperm protoplasts, was of the same order as that found in coleoptile derived protoplasts. No significant difference in expression was found for a given tissue between cv. Bomi and its mutant Risø 1508.
Type | Code | Acronym | Leader | Title |
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Government of Spain | PB89-0190 | Unspecified | Pilar Carbonero | Caracterización molecular de los genes de sacarosa sintética en trigo y cebada y su expresión en respuesta a factores ambientales y hormonales |
Government of Spain | BIO88-0216 | Unspecified | Unspecified | Unspecified |
Item ID: | 54025 |
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DC Identifier: | https://oa.upm.es/54025/ |
OAI Identifier: | oai:oa.upm.es:54025 |
DOI: | 10.1007/BF00232377 |
Official URL: | https://link.springer.com/article/10.1007%2FBF00232377 |
Deposited by: | Biblioteca ETSI Agrónomos |
Deposited on: | 18 Feb 2019 07:59 |
Last Modified: | 18 Feb 2019 07:59 |