Resonant nanopillars as label-free optical biosensors

López Hernández, Ana ORCID:, Casquel del Campo, Rafael ORCID:, Holgado, Miguel, Cornago, Iñaki, Fernández, Fátima, Ciaurriz, Paula, Sanza Gutierrez, Francisco Javier, Santamaria Fernández, Beatriz ORCID:, Maigler López, Mª Victoria and Laguna Heras, Maria Fe ORCID: (2018). Resonant nanopillars as label-free optical biosensors. "SPIE Proceedings", v. 10488 ; pp. 1048814-1. ISSN 0277-786X.


Title: Resonant nanopillars as label-free optical biosensors
Item Type: Article
Título de Revista/Publicación: SPIE Proceedings
Date: 2018
ISSN: 0277-786X
Volume: 10488
Freetext Keywords: Biofunctionalization; Bragg Reflectors; Label-free; Multiplexing; Resonant nanopillars
Faculty: Centro de Tecnología Biomédica (CTB) (UPM)
Department: Física Aplicada e Ingeniería de Materiales
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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In recent works, it has been demonstrated the suitability of using resonant nanopillars (R-NPs) as biochemical. In this work, it has been shown the capability of the R-NPs to behave as label-free multiplexed biological sensors. Each R-NP is formed by silicon oxide (SiO2) and silicon nitride (Si3N4) Bragg reflectors and a central cavity of SiO2, and they are grouped into eight arrays called BICELLs, which are distributed on a single chip of quartz substrate for multiplexing measurements. For the biological sensing assessment, it was developed an immunoassay on the eight single BICELLs. The biofunctionalization process was performed by a silanization protocol based on 3-aminopropyltrymethoxysilane (APTMS) and glutaradheyde (GA) as a linker between APTMS and the IgG which acted as bioreceptor for the anti-IgG recognition. In this work, there were compared two forms of immobilization: on one hand by incubating the R-NPs under static drop of 50 ?g/mL and on the second hand by introducing the sensing chip in a flow cell with a continuous flow of the same concentration of IgG. The eight arrays of R-NPs or BICELLs were independently optically interrogated by a bundle of fiber connected to a spectrometer. The multiplexing analysis showed reproducibility among the BICELLs, suggesting the potentially of using R-NPs for multiplexed biosensors. Performance in the immobilization process apparently does not have a signification effect. However, the election of one method or another should be a commitment between time and resources.

Funding Projects

Development of a biosensor technology for environmental monitoring and disease prevention in aquaculture ensuring food safety
Horizon 2020
Point-of-care device based on KETs for diagnosis of food allergies
Government of Spain
Biochip kits basados en celdas biofotónicas y plataformas avanzadas de interrogación óptica
Government of Spain
Desarrollo y validación clínica de un dispositivo para el diagnóstico y seguimiento de las alergias y tolerancias alimentarias

More information

Item ID: 54827
DC Identifier:
OAI Identifier:
DOI: 10.1117/12.2288273
Official URL:
Deposited by: Memoria Investigacion
Deposited on: 01 Feb 2023 15:01
Last Modified: 01 Feb 2023 15:01
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