Citation
Vila Perello, Miguel and Sánchez Vallet, Andrea and García Olmedo, Francisco and Molina Fernández, Antonio and Andreu, David
(2003).
Synthetic and structural studies on Pyrularia pubera thionin: a single-residue mutation enhances activity against Gram-negative bacteria.
"Febs Letters", v. 536
(n. 1-3);
pp. 215-219.
ISSN 0014-5793.
https://doi.org/10.1016/S0014-5793(03)00053-X.
Abstract
The thionin from Pyrularia pubera (Pp-TH), a 47-residue peptide with four internal disulfide bonds, was efficiently produced by chemical synthesis. Its antimicrobial activity in vitro against several representative pathogens (EC50=0.3–3.0 μM) was identical to that of natural Pp-TH. This peptide has a unique Asp32 instead of the consensus Arg found in other thionins of the same family. In order to evaluate the effect of this mutation, the Arg32 analogue (Pp-TH(D32R)) was also synthesized and showed a significant increase in antibiotic activity against several Gram-negative bacteria, whereas it retained the same activity against other pathogens. The overall structure of Pp-TH(D32R) was maintained, though a slight decrease in the helical content of the peptide was observed.