Full text
Preview |
PDF
- Requires a PDF viewer, such as GSview, Xpdf or Adobe Acrobat Reader
Download (274kB) | Preview |
Vila Perello, Miguel, Sánchez Vallet, Andrea ORCID: https://orcid.org/0000-0002-3668-9503, García Olmedo, Francisco, Molina Fernández, Antonio
ORCID: https://orcid.org/0000-0003-3137-7938 and Andreu, David
(2003).
Synthetic and structural studies on Pyrularia pubera thionin: a single-residue mutation enhances activity against Gram-negative bacteria.
"Febs Letters", v. 536
(n. 1-3);
pp. 215-219.
ISSN 0014-5793.
https://doi.org/10.1016/S0014-5793(03)00053-X.
Title: | Synthetic and structural studies on Pyrularia pubera thionin: a single-residue mutation enhances activity against Gram-negative bacteria |
---|---|
Author/s: |
|
Item Type: | Article |
Título de Revista/Publicación: | Febs Letters |
Date: | February 2003 |
ISSN: | 0014-5793 |
Volume: | 536 |
Subjects: | |
Freetext Keywords: | Thionin; Antimicrobial peptide; Oxidative folding; Plant |
Faculty: | E.T.S.I. Agrónomos (UPM) [antigua denominación] |
Department: | Biotecnologia [hasta 2014] |
Creative Commons Licenses: | Recognition - No derivative works - Non commercial |
Preview |
PDF
- Requires a PDF viewer, such as GSview, Xpdf or Adobe Acrobat Reader
Download (274kB) | Preview |
The thionin from Pyrularia pubera (Pp-TH), a 47-residue peptide with four internal disulfide bonds, was efficiently produced by chemical synthesis. Its antimicrobial activity in vitro against several representative pathogens (EC50=0.3–3.0 μM) was identical to that of natural Pp-TH. This peptide has a unique Asp32 instead of the consensus Arg found in other thionins of the same family. In order to evaluate the effect of this mutation, the Arg32 analogue (Pp-TH(D32R)) was also synthesized and showed a significant increase in antibiotic activity against several Gram-negative bacteria, whereas it retained the same activity against other pathogens. The overall structure of Pp-TH(D32R) was maintained, though a slight decrease in the helical content of the peptide was observed.
Item ID: | 5805 |
---|---|
DC Identifier: | https://oa.upm.es/5805/ |
OAI Identifier: | oai:oa.upm.es:5805 |
DOI: | 10.1016/S0014-5793(03)00053-X |
Official URL: | http://www.sciencedirect.com/science/article/pii/S... |
Deposited by: | Memoria Investigacion |
Deposited on: | 26 Jan 2011 11:01 |
Last Modified: | 20 Apr 2016 14:31 |