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Moreno, Manuel and Segura, Ana and García Olmedo, Francisco (1994). Pseudothionin-St1. A potato peptide active against potato pathogens.. "The FEBS Journal. European Journal of Biochemistry", v. 223 (n. 1); pp. 135-139. ISSN 1742-4658. https://doi.org/10.1111/j.1432-1033.1994.tb18974.x.
Title: | Pseudothionin-St1. A potato peptide active against potato pathogens. |
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Author/s: |
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Item Type: | Article |
Título de Revista/Publicación: | The FEBS Journal. European Journal of Biochemistry |
Date: | July 1994 |
ISSN: | 1742-4658 |
Volume: | 223 |
Subjects: | |
Faculty: | E.T.S.I. Agrónomos (UPM) [antigua denominación] |
Department: | Biotecnologia [hasta 2014] |
Creative Commons Licenses: | Recognition - No derivative works - Non commercial |
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A 5-kDa polypeptide, pseudothionin Solanum tuberosum 1(Pth-St1), which was active against Clavibacter michiganensis subspecies sepedonicus, a bacterial pathogen of potatoes, has been purified from the buffer-insoluble fraction of potato tubers by salt extraction and HPCL. Pth-St1 was also active against other potato pathogens tested (Pseudomonas solanacearum and Fusarium solani). The N-terminal amino acid sequence of this peptide was identical (except for a N/H substitution at position 2) to that deduced from a previously reported cDNA sequence (EMBL accession number X-13180), which had been misclassified as a Bowman-Birk protease inhibitor. Pth-St1 did not inhibit either trypsin or insect α-amylase activities, and, in contrast with true thionins, did not affect cell-free protein synthesis or β-glucuronidase activity. Northern-blot and tissue-print analyses showed that steady-state mRNA levels were highest in flowers (especially in petals), followed by tubers (especially in the epidermal cell layers and in leaf primordia), stems and leaves. Infection of leaves with a bacterial pathogen suspended in 10 mM MgCl2 switched off the gene, whereas mock inoculation with 10 mM MgCl2 alone induced higher mRNA levels.
Item ID: | 5862 |
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DC Identifier: | https://oa.upm.es/5862/ |
OAI Identifier: | oai:oa.upm.es:5862 |
DOI: | 10.1111/j.1432-1033.1994.tb18974.x |
Official URL: | http://onlinelibrary.wiley.com/doi/10.1111/j.1432-... |
Deposited by: | Memoria Investigacion |
Deposited on: | 02 Feb 2011 10:45 |
Last Modified: | 20 Apr 2016 14:34 |