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Effects of thionins on β-glucuronidase in vitro and in plant protoplasts
Citation
Diaz Rodriguez, Isabel and Carmona Quiles, Maria Jose and García Olmedo, Francisco (1992). Effects of thionins on β-glucuronidase in vitro and in plant protoplasts. "Febs Letters", v. 296 (n. 3); pp. 279-282. ISSN 0014-5793. https://doi.org/10.1016/0014-5793(92)80304-Y.
Description
| Title: | Effects of thionins on β-glucuronidase in vitro and in plant protoplasts |
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| Author/s: |
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| Item Type: | Article |
| Título de Revista/Publicación: | Febs Letters |
| Date: | January 1992 |
| ISSN: | 0014-5793 |
| Volume: | 296 |
| Subjects: | |
| Freetext Keywords: | β-Glucuronidase; Thionin; Tobacco protoplast; Transient expression |
| Faculty: | E.T.S.I. Agrónomos (UPM) [antigua denominación] |
| Department: | Biotecnologia [hasta 2014] |
| Creative Commons Licenses: | Recognition - No derivative works - Non commercial |
Abstract
Thionins cause the irreversible inactivation of β-glucuronidase (GUS) in vitro in a dose- and time-dependent manner. The enzyme is also sensitive to externally added thionins when expressed in the cytoplasmic compartment of tobacco protoplasts transformed with the Gus gene under the 35S promoter of the cauliflower mosaic virus. In protoplasts transformed with the Gus gene fused to a signal peptide, where GUS is translocated into the lumen of the endoplasmic reticulum, the activity is significantly increased both by externally-added and by transiently-expressed thionin, suggesting that it interferes with GUS secretion
More information
| Item ID: | 5876 |
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| DC Identifier: | https://oa.upm.es/5876/ |
| OAI Identifier: | oai:oa.upm.es:5876 |
| DOI: | 10.1016/0014-5793(92)80304-Y |
| Official URL: | http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T36-44XN036-7C&_user=885385&_coverDate=01/27/1992&_rdoc=1&_fmt=high&_orig=search&_origin=search&_sort=d&_docanchor=&view=c&_searchStrId=1628856885&_rerunOrigin=google&_acct=C000047350&_version=1&_ |
| Deposited by: | Memoria Investigacion |
| Deposited on: | 03 Feb 2011 08:51 |
| Last Modified: | 20 Apr 2016 14:34 |
