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Nigrosine staining of wheat endosperm proteolipid patterns on starch gels
Citation
Aragoncillo Ballesteros, Cipriano and Rodriguez Loperena, M.A. and Carbonero Zalduegui, Pilar and García Olmedo, Francisco (1975). Nigrosine staining of wheat endosperm proteolipid patterns on starch gels. "Analytical biochemistry", v. 63 (n. 2); pp. 603-606. ISSN 0003-2697. https://doi.org/10.1016/0003-2697(75)90387-5.
Description
| Title: | Nigrosine staining of wheat endosperm proteolipid patterns on starch gels |
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| Author/s: |
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| Item Type: | Article |
| Título de Revista/Publicación: | Analytical biochemistry |
| Date: | February 1975 |
| ISSN: | 0003-2697 |
| Volume: | 63 |
| Subjects: | |
| Faculty: | E.T.S.I. Agrónomos (UPM) [antigua denominación] |
| Department: | Biotecnologia [hasta 2014] |
| Creative Commons Licenses: | Recognition - No derivative works - Non commercial |
Abstract
We have previously characterized a group of proteolipids from wheat endosperm, designated CM proteins, which are soluble in chloroformmethanol (2:1, v/v) and have a molecular weight lower than 25,000 daltons (1-3). These have been also studied by Redman and Ewart (4). The CM proteins are suitably fractionated into several components by starch gel electrophoresis at pH 3.2 (1). A sensitive staining procedure was required in connection with genetic studies of these proteins because phenotypes had to be ascertained in small endosperm fractions dissected without impairing normal germination and plant development. We report here on Nigrosine staining conditions for CM proteins under which high sensitivity and selectivity are achieved
More information
| Item ID: | 5962 |
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| DC Identifier: | https://oa.upm.es/5962/ |
| OAI Identifier: | oai:oa.upm.es:5962 |
| DOI: | 10.1016/0003-2697(75)90387-5 |
| Official URL: | http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6W9V-4DYM9HN-13B&_user=10&_coverDate=02/28/1975&_rdoc=37&_fmt=high&_orig=browse&_origin=browse&_zone=rslt_list_item&_srch=doc-info(%23toc%236692%231975%23999369997%23534080%23FLP%23display%23Volume |
| Deposited by: | Memoria Investigacion |
| Deposited on: | 14 Feb 2011 08:49 |
| Last Modified: | 20 Apr 2016 14:38 |
