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Expression of the alpha-thionin gene from barley in tobacco confers enhanced resistance to bacterial pathogens
Citation
Carmona Quiles, Maria Jose and Molina Fernández, Antonio and Fernandez, Jose A. and Lopez Fando, J.J. and García Olmedo, Francisco (1993). Expression of the alpha-thionin gene from barley in tobacco confers enhanced resistance to bacterial pathogens. "Plant Journal", v. 3 (n. 3); pp. 457-462. ISSN 0960-7412. https://doi.org/10.1046/j.1365-313X.1993.t01-24-00999.x.
Description
| Title: | Expression of the alpha-thionin gene from barley in tobacco confers enhanced resistance to bacterial pathogens |
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| Author/s: |
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| Item Type: | Article |
| Título de Revista/Publicación: | Plant Journal |
| Date: | March 1993 |
| ISSN: | 0960-7412 |
| Volume: | 3 |
| Subjects: | |
| Faculty: | E.T.S.I. Agrónomos (UPM) [antigua denominación] |
| Department: | Biotecnologia [hasta 2014] |
| Creative Commons Licenses: | Recognition - No derivative works - Non commercial |
Abstract
Thionins are cysteine-rich, 5 kDa polypeptides which are toxic to plant pathogens in vitro. Expression of the gene encoding alpha-thionin from barley endosperm, under the 35S promoter from cauliflower mosaic virus, conferred to transgenic tobacco enhanced resistance to the bacterial plant pathogens Pseudomonas syringae pv. tabaci 153 and P. syringae pv. syringae. The barley alpha-thionin gene, which has two introns, was correctly spliced in tobacco. The alpha-thionin in transgenic plants had the expected mobility in the gradient, when separated by high- performance liquid chromatography, reacted with monospecific antibodies and showed the expected antibiotic properties in vitro
More information
| Item ID: | 6032 |
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| DC Identifier: | https://oa.upm.es/6032/ |
| OAI Identifier: | oai:oa.upm.es:6032 |
| DOI: | 10.1046/j.1365-313X.1993.t01-24-00999.x |
| Official URL: | http://onlinelibrary.wiley.com/doi/10.1046/j.1365-... |
| Deposited by: | Memoria Investigacion |
| Deposited on: | 16 Feb 2011 12:03 |
| Last Modified: | 20 Apr 2016 14:40 |
