Citation
García Olmedo, Francisco and Salcedo Duran, Gabriel and Aragoncillo Ballesteros, Cipriano and Sánchez-Monge Laguna de Rins, Rosa and Collada Collada, Maria Carmen and Gómez, Luis
(1988).
Two-dimensional electrophoresis as a tool for structural and genetic studies of seed proteins from Poaceae and Fagaceae..
"Electrophoresis", v. 9
(n. 11);
pp. 719-727.
ISSN 0173-0835.
https://doi.org/10.1002/elps.1150091107.
Abstract
The application of two-dimensional electrophoretic procedures to structural and genetic studies of seed proteins from Poaceae (including the cultivated cereals) and Fagaceae is described. The following related problems have been considered: covalent and non-covalent association of protein subunits in multiple oligomeric structures; chromosomal locations of genes encoding seed proteins; quantitation of gene products in relation to gene expression and regulation; purification of protein components to study their homology relationships and in vitro activities; evolutionary and phylogenetic relationships; identification of genetic stocks. Isoelectric focusing, pore-gradient electrophoresis, electrophoresis at different pH's, are among the separation procedures used in the first dimension, whereas sodium dodecyl sulfate-polyacrylamide gel electrophoresis and starch-gel electrophoresis at acid pH have been the preferred second-dimensional methods. Dissociating conditions (sodium dodecyl sulfate, Nonidet P-40, or urea) and reducing conditions (2-mercaptoethanol) have been used when required