Superoxide dismutase mimics improves semen quality during chilled preservation of rabbit spermatozoa

Sakr, Osama Galal and Gad, Ahmed and Rodríguez Francisco, María and García Rebollar, Pilar and Millán Pastor, Pilar (2019). Superoxide dismutase mimics improves semen quality during chilled preservation of rabbit spermatozoa. "Livestock Science", v. 221 ; pp. 70-76. ISSN 1871-1413. https://doi.org/10.1016/j.livsci.2019.01.015.

Description

Title: Superoxide dismutase mimics improves semen quality during chilled preservation of rabbit spermatozoa
Author/s:
  • Sakr, Osama Galal
  • Gad, Ahmed
  • Rodríguez Francisco, María
  • García Rebollar, Pilar
  • Millán Pastor, Pilar
Item Type: Article
Título de Revista/Publicación: Livestock Science
Date: March 2019
ISSN: 1871-1413
Volume: 221
Subjects:
Freetext Keywords: Antioxidants; Rabbits; Semen preservation
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Producción Agraria
Creative Commons Licenses: Recognition - No derivative works - Non commercial

Full text

[img]
Preview
PDF - Requires a PDF viewer, such as GSview, Xpdf or Adobe Acrobat Reader
Download (2MB) | Preview

Abstract

In rabbit farms, artificial insemination is usually undertaken using semen preserved around 18 ºC. However, increased reactive oxygen species levels during preservation produce sperm dysfunction. Our aim was to evaluate the effect of adding superoxide dismutase (SOD) antioxidant mimics (Tempo and TempoL) to the extenders of rabbit semen on quality parameters, total lipid peroxidation (LPO) and SOD level. Ejaculates from 12 sexually mature males were diluted with an extender containing 0 (control), 0.5, 1 or 2 mM of Tempo or TempoL, respectively. Semen samples were cooled till 18 ºC and stored for 0, 6 or 24 h. Sperm motility, velocity parameters, viability, plasma membrane integrity, SOD activity and level of thiobarbituric acid reactive substances (TBARS) were determined. Results showed that, in general, SOD antioxidant mimics significantly improved sperm motility compared to control after 6 and 24 h storage. Moreover, at 24 h, Tempo treatments showed motility rates higher than 80% whilst the control group showed the lowest motility rate among all treatments (58.5%, P < 0.05). Sperm viability showed no significant differences between treatments at 6 h and 24 h of storage. At 24 h, most SOD antioxidant mimics treatments significantly improved both curvilinear and mean velocity parameters, but only Tempo treatments improved linear velocity parameter. Also, SOD mimics protected sperm cells decreasing TBARS concentration at 6 and 24 h compared to 0 h. In conclusion, the addition of SOD antioxidant mimics during conservation at 18 ºC and storage of semen until 24 h reduces lipid peroxidation and preserves rabbit semen quality.

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainAGL2011-23822UnspecifiedPilar García RebollarMejora del rendimiento reproductivo en conejas primíparas sometidas a ritmos extensivos utilizando dietas ricas en ácidos grasos poliinsaturados y control de lactación
Government of SpainAGL2015-65572-C2UnspecifiedUnspecifiedEfecto de la restricción alimentaria materna en la respuesta ovárica y embrionaria del papel del factor de crecimiento nervioso (NGF) tras su caracterización en el conejo
Madrid Regional GovernmentS2013/ABI-2913MEDGANUnspecifiedTecnologías de manejo, nutrición y genética para optimizar la producción de alimentos de origen animal característicos de la dieta mediterránea

More information

Item ID: 63655
DC Identifier: https://oa.upm.es/63655/
OAI Identifier: oai:oa.upm.es:63655
DOI: 10.1016/j.livsci.2019.01.015
Official URL: https://www.sciencedirect.com/science/article/pii/S1871141319300708
Deposited by: Memoria Investigacion
Deposited on: 18 Nov 2020 08:50
Last Modified: 18 Nov 2020 08:50
  • Logo InvestigaM (UPM)
  • Logo GEOUP4
  • Logo Open Access
  • Open Access
  • Logo Sherpa/Romeo
    Check whether the anglo-saxon journal in which you have published an article allows you to also publish it under open access.
  • Logo Dulcinea
    Check whether the spanish journal in which you have published an article allows you to also publish it under open access.
  • Logo de Recolecta
  • Logo del Observatorio I+D+i UPM
  • Logo de OpenCourseWare UPM