Taxonomic and functional analysis of soil microbial communities in a mining site across a metal(loid) contamination gradient

Navas Vásquez, Mariela and Pérez Esteban, Javier and Torres Lacruz, Miguel Ángel and Hontoria Fernández, Chiquinquirá and Moliner Aramendia, Ana María (2021). Taxonomic and functional analysis of soil microbial communities in a mining site across a metal(loid) contamination gradient. "European Journal of Soil Science", v. 72 (n. 3); pp. 1190-1205. ISSN 1351-0754. https://doi.org/10.1111/ejss.12979.

Description

Title: Taxonomic and functional analysis of soil microbial communities in a mining site across a metal(loid) contamination gradient
Author/s:
  • Navas Vásquez, Mariela
  • Pérez Esteban, Javier
  • Torres Lacruz, Miguel Ángel
  • Hontoria Fernández, Chiquinquirá
  • Moliner Aramendia, Ana María
Item Type: Article
Título de Revista/Publicación: European Journal of Soil Science
Date: May 2021
ISSN: 1351-0754
Volume: 72
Subjects:
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Otro
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Soil microorganisms surviving in mining sites have developed metal resistance mechanisms to biotransform metals, and their use as biofertilizers can promote plant growth and reduce metal toxicity, thus improving the phytoremediation efficiency in contaminated soils. We analysed through a whole-metagenome shotgun sequencing approach the composition, diversity and function of the microbial communities of a contaminated mine soil along a gradient of metal(loid)s (As, Cu, Fe, Mn, Pb, Zn) in order to identify tolerant species carrying metal-resistance functional genes that can be used in association with plants for phytoremediation strategies. Soil samples were collected across three areas with different levels of metal contamination (not affected, moderately contaminated and highly contaminated areas) from an abandoned copper mine. We found that the relative abundance of Proteobacteria increased in highly contaminated areas, especially the genus Bradyrhizobium, whereas Actinobacteria dominated in the not affected area. Archaea (Euryarchaeota), with the most abundant genera Haloarcula, Halobacterium and Halorubrum, predominated in the moderately contaminated soil. As regards to fungi, Basidiomycota did not exhibit differences between areas, while Ascomycota and its genus Aspergillus increased in areas with low metal concentrations. Metal-resistance genes associated to Fe (acn, furA, dpsA), Cu (cop-unnamed, copF, actP, copA, mmco, cutO) and As (arsT, arsC, aioA/aoxB) metabolism were the most abundant and were affected by the gradient of soil contamination. Those associated to Cu had higher abundance in the most contaminated areas, whereas As and Fe genes were more abundant in the least contaminated. Bradyrhizobium diazoefficiens, Pseudomonas aeruginosa, Halorubrum trapanicum, Aspergillus fumigatus, Aspergillus fischeri were abundant in the most contaminated soils and are carriers of metal-resistance genes. These species are promising tools for their use as biofertilizers with phytoremediation plants in contaminated mining sites

Funding Projects

TypeCodeAcronymLeaderTitle
Madrid Regional GovernmentS2018/BAA-4330AGRISOST-CMUnspecifiedUnspecified
Government of SpainCTM2013-47874-C2-1-RUnspecifiedUnspecifiedBiorremediación de ecosistemas contaminados por metales pesados y contaminantes orgánicos persistentes empleando sustratos y enmiendas ecoeficientes

More information

Item ID: 63813
DC Identifier: https://oa.upm.es/63813/
OAI Identifier: oai:oa.upm.es:63813
DOI: 10.1111/ejss.12979
Official URL: https://onlinelibrary.wiley.com/journal/13652389
Deposited by: Memoria Investigacion
Deposited on: 10 May 2021 08:06
Last Modified: 10 May 2021 08:06
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