Transit Peptides From Photosynthesis-Related Proteins Mediate Import of a Marker Protein Into Different Plastid Types and Within Different Species

Eséverri Sabaté, Álvaro and Baysal, Can and Medina Piles, Vicente and Capell Capell, Teresa and Christou, Paul and Rubio Herrero, Luis Manuel and Caro Bernat, Elena (2020). Transit Peptides From Photosynthesis-Related Proteins Mediate Import of a Marker Protein Into Different Plastid Types and Within Different Species. "Frontiers in Plant Science", v. 11 ; p. 560701. ISSN 1664-462X. https://doi.org/10.3389/fpls.2020.560701.

Description

Title: Transit Peptides From Photosynthesis-Related Proteins Mediate Import of a Marker Protein Into Different Plastid Types and Within Different Species
Author/s:
  • Eséverri Sabaté, Álvaro
  • Baysal, Can
  • Medina Piles, Vicente
  • Capell Capell, Teresa
  • Christou, Paul
  • Rubio Herrero, Luis Manuel
  • Caro Bernat, Elena
Item Type: Article
Título de Revista/Publicación: Frontiers in Plant Science
Date: September 2020
ISSN: 1664-462X
Volume: 11
Subjects:
Freetext Keywords: plastid targeting; recombinant protein; plastid import; transit peptide; rice; crops; synthetic biology; biotechnology
Faculty: Centro de Investigación en Biotecnología y Genómica de Plantas (CBGP) (UPM)
Department: Otro
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Nucleus-encoded plastid proteins are synthesized as precursors with N-terminal targeting signals called transit peptides (TPs), which mediate interactions with the translocon complexes at the outer (TOC) and inner (TIC) plastid membranes. These complexes exist in multiple isoforms in higher plants and show differential specificity and tissue abundance. While some show specificity for photosynthesis-related precursor proteins, others distinctly recognize non-photosynthetic and housekeeping precursor proteins. Here we used TPs from four Arabidopsis thaliana proteins, three related to photosynthesis (chlorophyll a/b binding protein, Rubisco activase) and photo-protection (tocopherol cyclase) and one involved in the assimilation of ammonium into amino-acids, and whose expression is most abundant in the root (ferredoxin dependent glutamate synthase 2), to determine whether they were able to mediate import of a nuclear-encoded marker protein into plastids of different tissues of a dicot and a monocot species. In A. thaliana, import and processing efficiency was high in all cases, while TP from the rice Rubisco small chain 1, drove very low import in Arabidopsis tissues. Noteworthy, our results show that Arabidopsis photosynthesis TPs also mediate plastid import in rice callus, and in leaf and root tissues with almost a 100% efficiency, providing new biotechnological tools for crop improvement strategies based on recombinant protein accumulation in plastids by the expression of nuclear-encoded transgenes.

More information

Item ID: 64189
DC Identifier: https://oa.upm.es/64189/
OAI Identifier: oai:oa.upm.es:64189
DOI: 10.3389/fpls.2020.560701
Official URL: https://www.frontiersin.org/articles/10.3389/fpls.2020.560701/
Deposited by: Memoria Investigacion
Deposited on: 21 Apr 2021 07:21
Last Modified: 21 Apr 2021 07:21
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