Characterization of novel developmental cues in Arabidopsis roots

Serrano Ron, Laura and Cabrera Chaves, Javier and Moreno Risueño, Miguel Ángel (2019). Characterization of novel developmental cues in Arabidopsis roots. In: "XXIII Meeting of the Spanish Society of Plant Physiology/XVI Spanish Portuguese Congress of Plant Physiology", 26/06/2019-28/06/2019, Pamplona, España. p. 1.

Description

Title: Characterization of novel developmental cues in Arabidopsis roots
Author/s:
  • Serrano Ron, Laura
  • Cabrera Chaves, Javier
  • Moreno Risueño, Miguel Ángel
Item Type: Presentation at Congress or Conference (Other)
Event Title: XXIII Meeting of the Spanish Society of Plant Physiology/XVI Spanish Portuguese Congress of Plant Physiology
Event Dates: 26/06/2019-28/06/2019
Event Location: Pamplona, España
Title of Book: Libro de Abstracts de la XXIII Reunión de la Sociedad Española de Fisiología Vegetal y XVI Spanish Portuguese Congress of Plant Physiology
Date: June 2019
Subjects:
Faculty: Centro de Investigación en Biotecnología y Genómica de Plantas (CBGP) (UPM)
Department: Otro
Creative Commons Licenses: Recognition - No derivative works - Non commercial

Full text

[img]
Preview
PDF - Requires a PDF viewer, such as GSview, Xpdf or Adobe Acrobat Reader
Download (428kB) | Preview

Abstract

The root system and its functionality is essential for survival of terrestrial plants. Lateral root (LR) formation is a plastic process regulating root system architecture, although locations where LRs may form are pre-patterned by the Lateral Root Clock. Under standard growth conditions, LRs form as repeated units along the root primary axis1,2. The Lateral Root Clock was first defined using the synthetic auxin-response promoter DR5, which pulses rhythmically about every 5 hours in a zone close from the root tip: the Oscillation Zone (OZ). Further analyses of this region showed changes in the expression of thousands of genes that fluctuated in or out of phase with DR53. As cells exit the OZ, oscillations cease and maxima in auxin response (peak of oscillations) are followed by permanent expression of DR5 in specific sites, called Pre-Branch Sites (PBS). At these sites, LR initiation will eventually occur3,4. The OZ arises as a key developmental zone for LR pre-patterning. It is unknown how this zone is positioned and delimited. arf7arf19 mutants do not form LRs, however present constitutive non-oscillating expression of DR5 in the OZ3. Thus, DR5 can be used in arf7arf19 mutant to define the OZ. A genetic screen using Ethyl Methanesulfonate (EMS) was performed in arf7arf19 mutants carrying the DR5:Luciferase marker. Mutagenized F2 populations were searched for alterations in DR5 expression in the OZ. We aimed to find mutants with expanded marker expression, as expansion of the OZ would indicate the existence of a repressing signal, likely unknown, with an antagonistic role to auxin. Here we show the different phenotypes of mutants we have found. These were classified in three groups: A (expanded OZ), B (higher DR5 intensity in the OZ) and C (subtle changes in DR5 but increased LR formation). In addition, we crossed these mutants back to the wild type to remove arf7 and arf19 along with other undesired mutations. Segregating populations were analyzed to determine the effect of mutations in OZ positioning and PBS formation, which are indicative of their putative role as developmental cues. Based on these data, we will prioritize mutants and proceed to map them

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainBFU2016-80315-PUnspecifiedUnspecifiedNuevos mecanismos que especifican y regeneran órganos postembrionarios en la raíz de Arabidopsis
Government of SpainBES-2017-080155UnspecifiedUnspecifiedUnspecified

More information

Item ID: 64704
DC Identifier: https://oa.upm.es/64704/
OAI Identifier: oai:oa.upm.es:64704
Deposited by: Memoria Investigacion
Deposited on: 22 Apr 2021 08:41
Last Modified: 22 Apr 2021 08:41
  • Logo InvestigaM (UPM)
  • Logo GEOUP4
  • Logo Open Access
  • Open Access
  • Logo Sherpa/Romeo
    Check whether the anglo-saxon journal in which you have published an article allows you to also publish it under open access.
  • Logo Dulcinea
    Check whether the spanish journal in which you have published an article allows you to also publish it under open access.
  • Logo de Recolecta
  • Logo del Observatorio I+D+i UPM
  • Logo de OpenCourseWare UPM