Estudio del efecto de extractos de origen alimentario sobre el metabolismo de la microbiota intestinal

Barajas Mascaraque, Laura (2020). Estudio del efecto de extractos de origen alimentario sobre el metabolismo de la microbiota intestinal. Proyecto Fin de Carrera / Trabajo Fin de Grado, E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM), Madrid, España.

Description

Title: Estudio del efecto de extractos de origen alimentario sobre el metabolismo de la microbiota intestinal
Author/s:
  • Barajas Mascaraque, Laura
Contributor/s:
  • Simó Ruiz, Carolina
  • Bañuelos Bernabe, Maria Antonia
Item Type: Final Project
Degree: Grado en Biotecnología
Date: July 2020
Subjects:
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Nowadays, cardiovascular diseases are considered to be one of the leading causes of mortality and morbidity worldwide. Among this group of diseases, atherosclerosis stands out as one of the most frequent causes of coronary heart failure. Atherosclerosis is an inflammatory illness triggered by the interaction of several metabolic and immune factors which lead to the thickening of the arteries’ walls, blocking the flow of the blood and consequently causing organs ischemia. The risk factors which increase the probability of developing atherosclerosis include smoking, bad nutrition habits or sedentarism. Nevertheless, it has been recently demonstrated that our intestinal microbiota plays an important role in the development of atherosclerosis. Several studies have revealed that some bacteria from the intestinal microbiota are able to transform certain dietary components, such us phosphatidylcholine, L-carnitine or choline, into trimethylamine, a microbial metabolite which is then absorbed by the intestine and converted into trimethylamine N-oxide in the liver. This trimethylamine N-oxide has been found to be related with the development and progression of atherosclerosis. With the objective of reducing the incidence and development of the illness, various studies have identified new possible nutritional factors and constituents with antiatherogenic activity. Moreover, although there are scientific evidences which suggest that certain natural extracts may be able to influence the intestinal microbial metabolism and reduce atherosclerosis progression, none of them have been proven to act as inhibitors of the microbial production of TMA from dietary L-carnitine. For these reasons, the present work is aimed to identify natural extracts with the ability to inhibit the bacterial Klebsiella pneumoniae L-carnitine catabolism, and therefore the TMA production. To achieve this goal, a high-throughput screening method was used based on the reduction of the resazurin reagent, which allowed the monitorization of K. pneumoniaes’ metabolic activity when treated with the different extracts. The results showed that the extracts obtained with hexane from garlic and purple onion were able to prevent the usage of the L-carnitine present in the medium as the only carbon source by K. pneumoniae. For this reason, although further studies should be made, these extracts could be considered possible inhibitors of the enzyme carnitine monooxygenase. These findings will be of interest for the development of new nutritional and personalized strategies which will be used for the prevention of cardiovascular diseases.

More information

Item ID: 66786
DC Identifier: https://oa.upm.es/66786/
OAI Identifier: oai:oa.upm.es:66786
Deposited by: Biblioteca ETSI Agrónomos
Deposited on: 19 Apr 2021 10:14
Last Modified: 19 Jun 2021 22:30
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