Application of the MSAP technique to evaluate epigenetic changes in plant conservation

González Benito, Elena and Ibañez Ruiz, Miguel Ángel and Pirredda, Michela and Mira Pérez, Sara and Martín Fernández, María Carmen (2020). Application of the MSAP technique to evaluate epigenetic changes in plant conservation. "International Journal of Molecular Sciences", v. 21 (n. 20); p. 7459. ISSN 1422-0067. https://doi.org/10.3390/ijms21207459.

Description

Title: Application of the MSAP technique to evaluate epigenetic changes in plant conservation
Author/s:
  • González Benito, Elena
  • Ibañez Ruiz, Miguel Ángel
  • Pirredda, Michela
  • Mira Pérez, Sara
  • Martín Fernández, María Carmen
Item Type: Article
Título de Revista/Publicación: International Journal of Molecular Sciences
Date: October 2020
ISSN: 1422-0067
Volume: 21
Subjects:
Freetext Keywords: cryopreservation; DNA methylation; multinomial model; plant tissue culture; seed storage
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Epigenetic variation, and particularly DNA methylation, is involved in plasticity and responses to changes in the environment. Conservation biology studies have focused on the measurement of this variation to establish demographic parameters, diversity levels and population structure to design the appropriate conservation strategies. However, in ex situ conservation approaches, the main objective is to guarantee the characteristics of the conserved material (phenotype and epi-genetic). We review the use of the Methylation Sensitive Amplified Polymorphism (MSAP) technique to detect changes in the DNA methylation patterns of plant material conserved by the main ex situ plant conservation methods: seed banks, in vitro slow growth and cryopreservation. Comparison of DNA methylation patterns before and after conservation is a useful tool to check the fidelity of the regenerated plants, and, at the same time, may be related with other genetic variations that might appear during the conservation process (i.e., somaclonal variation). Analyses of MSAP profiles can be useful in the management of ex situ plant conservation but differs in the approach used in the in situ conservation. Likewise, an easy-to-use methodology is necessary for a rapid interpretation of data, in order to be readily implemented by conservation managers.

Funding Projects

TypeCodeAcronymLeaderTitle
Government of SpainAGL2015-64899-C2-1-PUnspecifiedUnspecifiedDaño oxidativo y su efecto sobre el deterioro y la calidad del germoplasma vegetal conservado ex situ

More information

Item ID: 68394
DC Identifier: https://oa.upm.es/68394/
OAI Identifier: oai:oa.upm.es:68394
DOI: 10.3390/ijms21207459
Official URL: https://www.mdpi.com/1422-0067/21/20/7459
Deposited by: Memoria Investigacion
Deposited on: 09 Sep 2021 09:32
Last Modified: 09 Sep 2021 09:33
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