Estudio de la interacción entre plaquetas y PBMCs (Células mononucleares de sangre periférica) en pacientes con alergias respiratorias

Ciudad Espinosa, Ana (2021). Estudio de la interacción entre plaquetas y PBMCs (Células mononucleares de sangre periférica) en pacientes con alergias respiratorias. Proyecto Fin de Carrera / Trabajo Fin de Grado, E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM), Madrid.

Description

Title: Estudio de la interacción entre plaquetas y PBMCs (Células mononucleares de sangre periférica) en pacientes con alergias respiratorias
Author/s:
  • Ciudad Espinosa, Ana
Contributor/s:
  • Peinado Lozano, Ana Belén
  • Izquierdo Álvarez, Elena
Item Type: Final Project
Degree: Grado en Biotecnología
Date: June 2021
Subjects:
Faculty: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
Department: Biotecnología - Biología Vegetal
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Allergy is already the most prevalent chronic disease of the 21st century. Allergic diseases have experienced a constant increase both in prevalence and severity in the last decades. Understanding the causes underlying this increase and developing new biomarkers strategies to monitor patient evolution, predict and choose the best intervention strategies is increasingly needed. In previous projects, it has been observed that modifications on platelets are associated with the progression of severity degree in allergy ; this proposal hypothesizes that platelet-immune cell interactions could be altered in severe patients, influencing its lymphocytes and monocytes’ bioenergetic profile and its immune function. These changes could be used in order to better understand allergic pathology and find out new therapeutical targets. Thus, the main objective of this project was to elaborate a reproducible protocol to study the bioenergetic and transcriptomic profile of lymphocytes and monocytes’ (obtained from frozen and fresh patient samples) and the potential role of platelets as modulators of the immune response depending on patients’ severity degree. The results confirmed that it is possible to analyse lymphocytes from frozen PBMCs, but not monocytes, because of their low recovery rate. It has been proven that platelets modulate the bioenergetic profile of these cells, and preliminary differences between the stimulation with plasma from healthy donors or from severe patients have been found: lymphocytes cultivated with severe patient plasma-poor-platelet (PPP) increase their maximal respiration and spare respiratory capacity in a higher rate than lymphocytes cultivated with healthy donor PPP, which is not observed in lymphocytes cultivated with plasma-rich-platelet (PRP). Moreover, differential gene expression of representative allergy response genes can be also appreciated in PBMCs after the stimulation with PPP and PRP, revealing the importance of platelets-PBMCs interaction.

More information

Item ID: 69328
DC Identifier: https://oa.upm.es/69328/
OAI Identifier: oai:oa.upm.es:69328
Deposited by: Biblioteca ETSI Agrónomos
Deposited on: 20 Dec 2021 14:22
Last Modified: 20 Dec 2021 14:22
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