title: Schizosaccharomyces selective differential media
creator: Benito Saez, Santiago
creator: Galvez Patón, Laura
creator: Palomero Rodriguez, Felipe
creator: Calderon Fernandez, Fernando
creator: Morata Barrado, Antonio
creator: Palmero Llamas, Daniel
creator: Suarez Lepe, Jose Antonio
subject: Agriculture
description: This study discusses the optimisation of a selectiv e and differential medium which would facilitate the isolation of Schizosaccharomyces (a genus with a low incidence compared to other microorganisms) to select individuals from this genus for industrial purposes, especially in light of the recent approval of the use of yeasts from this genus in the wine industry by the International Organisation of Vine and Wine, or to detect the presence of such yeasts, for those many authors who consider them food spoilers. To this end, we studied various selective differential agents based on the main thephysiological characteristics of this species, such as its high resistance to high concentrations of sugar, sulfur dioxide, sorbic acid, benzoic acid, acetic acid or malo ethanolic fermentation. This selective medium is based on the resistance of the genus to the antibiotic actidione and its high resistance to inhibitory agents such as benzoic acid compared to possible microorganisms which can give rise to false positive results. Malic acid was used as a differential fact or due to the ability of this genus to metabolise it to ethanol, which allows detecting of the degradation of this compound. Lastly, the medium was successfully used to isolate strains of Schizosaccharomyces pombe from honey.
publisher: E.U.I.T. Agrícola (UPM)
rights: https://creativecommons.org/licenses/by-nc-nd/3.0/es/
date: 2013
type: info:eu-repo/semantics/article
type: Article
source: African Journal of Microbiology Research, ISSN 1996-0808, 2013, Vol. 7, No. 24
type: PeerReviewed
format: application/pdf
language: eng
rights: info:eu-repo/semantics/openAccess
identifier: https://oa.upm.es/19114/