TY - JOUR SP - 3026 VL - 7 JF - African Journal of Microbiology Research UR - https://oa.upm.es/19114/ SN - 1996-0808 Y1 - 2013/// A1 - Benito Saez, Santiago A1 - Galvez Patón, Laura A1 - Palomero Rodriguez, Felipe A1 - Calderon Fernandez, Fernando A1 - Morata Barrado, Antonio A1 - Palmero Llamas, Daniel A1 - Suarez Lepe, Jose Antonio AV - public EP - 3036 ID - upm19114 PB - CSIRO Publishers TI - Schizosaccharomyces selective differential media N2 - This study discusses the optimisation of a selectiv e and differential medium which would facilitate the isolation of Schizosaccharomyces (a genus with a low incidence compared to other microorganisms) to select individuals from this genus for industrial purposes, especially in light of the recent approval of the use of yeasts from this genus in the wine industry by the International Organisation of Vine and Wine, or to detect the presence of such yeasts, for those many authors who consider them food spoilers. To this end, we studied various selective differential agents based on the main thephysiological characteristics of this species, such as its high resistance to high concentrations of sugar, sulfur dioxide, sorbic acid, benzoic acid, acetic acid or malo ethanolic fermentation. This selective medium is based on the resistance of the genus to the antibiotic actidione and its high resistance to inhibitory agents such as benzoic acid compared to possible microorganisms which can give rise to false positive results. Malic acid was used as a differential fact or due to the ability of this genus to metabolise it to ethanol, which allows detecting of the degradation of this compound. Lastly, the medium was successfully used to isolate strains of Schizosaccharomyces pombe from honey. IS - 24 ER -