eprintid: 19114
rev_number: 17
eprint_status: archive
userid: 1903
dir: disk0/00/01/91/14
datestamp: 2013-08-28 11:05:21
lastmod: 2023-02-27 06:31:32
status_changed: 2023-02-27 06:31:32
type: article
metadata_visibility: show
item_issues_count: 0
creators_name: Benito Saez, Santiago
creators_name: Galvez Patón, Laura
creators_name: Palomero Rodriguez, Felipe
creators_name: Calderon Fernandez, Fernando
creators_name: Morata Barrado, Antonio
creators_name: Palmero Llamas, Daniel
creators_name: Suarez Lepe, Jose Antonio
creators_id: santiago.benito@upm.es
creators_id: daniel.palmero@upm.es
title: Schizosaccharomyces selective differential media
ispublished: pub
subjects: agricultura
abstract: This study discusses the optimisation of a selectiv e and differential medium which would facilitate the isolation of Schizosaccharomyces (a genus with a low incidence compared to other microorganisms) to select individuals from this genus for industrial purposes, especially in light of the recent approval of the use of yeasts from this genus in the wine industry by the International Organisation of Vine and Wine, or to detect the presence of such yeasts, for those many authors who consider them food spoilers. To this end, we studied various selective differential agents based on the main thephysiological characteristics of this species, such as its high resistance to high concentrations of sugar, sulfur dioxide, sorbic acid, benzoic acid, acetic acid or malo ethanolic fermentation. This selective medium is based on the resistance of the genus to the antibiotic actidione and its high resistance to inhibitory agents such as benzoic acid compared to possible microorganisms which can give rise to false positive results. Malic acid was used as a differential fact or due to the ability of this genus to metabolise it to ethanol, which allows detecting of the degradation of this compound. Lastly, the medium was successfully used to isolate strains of Schizosaccharomyces pombe from honey.
date: 2013
date_type: published
publisher: CSIRO Publishers
full_text_status: public
publication: African Journal of Microbiology Research
volume: 7
number: 24
pagerange: 3026-3036
institution: Agricola
department: Produccion_Vegetal
refereed: TRUE
issn: 1996-0808
rights: by-nc-nd
citation:   Benito Saez, Santiago and Galvez Patón, Laura and Palomero Rodriguez, Felipe and Calderon Fernandez, Fernando and Morata Barrado, Antonio and Palmero Llamas, Daniel and Suarez Lepe, Jose Antonio  (2013).  Schizosaccharomyces selective differential media.   "African Journal of Microbiology Research", v. 7  (n. 24);   pp. 3026-3036.  ISSN 1996-0808.    
document_url: https://oa.upm.es/19114/1/INVE_MEM_2013_141779.pdf