eprintid: 29160 rev_number: 34 eprint_status: archive userid: 1903 dir: disk0/00/02/91/60 datestamp: 2014-07-29 17:21:38 lastmod: 2015-03-31 22:56:05 status_changed: 2014-07-29 17:21:38 type: article metadata_visibility: show item_issues_count: 0 creators_name: Corrales Ducuara, Alba Rocio creators_name: Nebauer, Sergio G. creators_name: Carrillo Gil, Laura creators_name: Fernandez Nohales, Pedro creators_name: Marqués, Jorge creators_name: Renau Morata, Begoña creators_name: Granel, Antonio creators_name: Pollmann, Stephan creators_name: Vicente Carbajosa, Jesus creators_name: Molina, Rosa Victoria creators_name: Medina Alcazar, Joaquin title: Characterization of tomato Cycling Dof Factors reveals conserved and new functions in the control of flowering time and abiotic stress responses. rights: by-nc-nd ispublished: pub subjects: biologia full_text_status: public abstract: DNA binding with One Finger (DOF) transcription factors are involved in multiple aspects of plant growth and development but their precise roles in abiotic stress tolerance are largely unknown. Here we report a group of five tomato DOF genes, homologous to Arabidopsis Cycling DOF Factors (CDFs), that function as transcriptional regulators involved in responses to drought and salt stress and flowering-time control in a gene-specific manner. SlCDF1?5 are nuclear proteins that display specific binding with different affinities to canonical DNA target sequences and present diverse transcriptional activation capacities in vivo. SlCDF1?5 genes exhibited distinct diurnal expression patterns and were differentially induced in response to osmotic, salt, heat, and low-temperature stresses. Arabidopsis plants overexpressing SlCDF1 or SlCDF3 showed increased drought and salt tolerance. In addition, the expression of various stress-responsive genes, such as COR15, RD29A, and RD10, were differentially activated in the overexpressing lines. Interestingly, overexpression in Arabidopsis of SlCDF3 but not SlCDF1 promotes late flowering through modulation of the expression of flowering control genes such as CO and FT. Overall, our data connect SlCDFs to undescribed functions related to abiotic stress tolerance and flowering time through the regulation of specific target genes and an increase in particular metabolites date_type: published date: 2013-01 publication: Journal of Experimental Botany volume: 65 number: 4 pagerange: 995-1012 id_number: 10.1093/jxb/ert451 institution: Biotecnologia_Genomica department: otro refereed: TRUE issn: 0022-0957 official_url: http://jxb.oxfordjournals.org/content/65/4/995.abstract citation: Corrales Ducuara, Alba Rocio and Nebauer, Sergio G. and Carrillo Gil, Laura and Fernandez Nohales, Pedro and Marqués, Jorge and Renau Morata, Begoña and Granel, Antonio and Pollmann, Stephan and Vicente Carbajosa, Jesus and Molina, Rosa Victoria and Medina Alcazar, Joaquin (2013). Characterization of tomato Cycling Dof Factors reveals conserved and new functions in the control of flowering time and abiotic stress responses.. "Journal of Experimental Botany", v. 65 (n. 4); pp. 995-1012. ISSN 0022-0957. https://doi.org/10.1093/jxb/ert451 . document_url: https://oa.upm.es/29160/1/INVE_MEM_2013_168207.pdf