TY  - JOUR
A1  - Cañón Beltrán, K.
A1  - Cajas, Y.N.
A1  - García García, R. M.
A1  - Lorenzo, P.L.
A1  - Carrera, R.
A1  - Garcia Rebollar, Pilar
A1  - Arias Álvarez, María
UR  - https://oa.upm.es/40169/
AV  - public
JF  - Animal Reproduction
TI  - Effect of epidermal growth factor on nuclear and cytoplasmic in vitro maruration of guinea pig oocytes
ID  - upm40169
SP  - 793
N2  - The guinea pig may represent an animal model for research on ovarian infertility and improvement of the in vitro 
maturation (IVM) conditions is needed in this species. The aim of the present work was to immunolocalize the Epidermal Growth Factor (EGF)-Receptor in the guinea pig ovaries and to study the effect of EGF on meiotic and cytoplasmic  maturation,  and  apoptotic  rate  in  cumulus-oocyte-co
mplexes  (COCs).  Immunohistochemistry  was performed in paraffined ovaries using a rabbit polyclonal antibody EGF-R (1:100; Santa Cruz Biotechnology) and the  ABC  Vector  Elite  kit  (Vector  Laboratories).  For  the  IVM,  COCs  were  collected  by  aspiration  of  follicles >700?m under a stereoscopic microscope.
SN  - 1806-9614
Y1  - 2015///
EP  - 793
VL  - 12
IS  - 3
ER  -