title: Increased insect resistance in transgenic wheat stably expressing trypsin inhibitor CMe
creator: Altpeter, Fredy
creator: Diaz Rodriguez, Isabel
creator: McAuslane, Heather
creator: Gaddour, Kamel
creator: Carbonero Zalduegui, Pilar
creator: Vasil, Indra K.
subject: Agriculture
subject: Biology
description: Proteinase inhibitors have been proposed to function as plant defence agents against herbivorous pests. We have introduced the barley trypsin inhibitor CMe (BTI-CMe) into wheat (Triticum aestivum L.) by biolistic bombardment of cultured immature embryos. Of the 30 independent transgenic wheat lines selected, 16 expressed BTI-CMe. BTI-CMe was properly transcribed and translated as indicated by northern and western blot, with a level of expression in transgenic wheat seeds up to 1.1% of total extracted protein. No expression was detected in untransformed wheat seeds. Functional integrity of BTI-CMe was confirmed by trypsin inhibitor activity assay. The significant reduction of the survival rate of the Angoumois grain moth (Sitotroga cerealella, Lepidoptera: Gelechiidae), reared on transgenic wheat seeds expressing the trypsin inhibitor BTI-CMe, compared to the untransformed control confirmed the potential of BTI-CMe for the increase of insect resistance. However, only early-instar larvae were inhibited in transgenic seeds and expression of BTI-CMe protein in transgenic leaves did not have a significant protective effect against leaf-feeding insects.
publisher: E.T.S. de Ingeniería Agronómica, Alimentaria y de Biosistemas (UPM)
rights: https://creativecommons.org/licenses/by-nc-nd/3.0/es/
date: 1999-01
type: info:eu-repo/semantics/article
type: Article
source: Molecular Breeding, ISSN 1380-3743, 1999-01, Vol. 5, No. 1
type: PeerReviewed
format: application/pdf
language: eng
relation: https://link.springer.com/article/10.1023/A:1009659911798
relation: info:eu-repo/grantAgreement/MINECO//BIO96-2303
rights: info:eu-repo/semantics/openAccess
relation: info:eu-repo/semantics/altIdentifier/doi/10.1023/A:10096599
identifier: https://oa.upm.es/53944/