eprintid: 53944 rev_number: 20 eprint_status: archive userid: 2457 dir: disk0/00/05/39/44 datestamp: 2019-02-12 07:00:23 lastmod: 2019-02-12 07:42:51 status_changed: 2019-02-12 07:42:51 type: article metadata_visibility: show creators_name: Altpeter, Fredy creators_name: Diaz Rodriguez, Isabel creators_name: McAuslane, Heather creators_name: Gaddour, Kamel creators_name: Carbonero Zalduegui, Pilar creators_name: Vasil, Indra K. creators_id: i.diaz@upm.es creators_id: p.carbonero@upm.es title: Increased insect resistance in transgenic wheat stably expressing trypsin inhibitor CMe publisher: Kluwer Academic Publishers rights: by-nc-nd ispublished: pub subjects: agricultura subjects: biologia full_text_status: public abstract: Proteinase inhibitors have been proposed to function as plant defence agents against herbivorous pests. We have introduced the barley trypsin inhibitor CMe (BTI-CMe) into wheat (Triticum aestivum L.) by biolistic bombardment of cultured immature embryos. Of the 30 independent transgenic wheat lines selected, 16 expressed BTI-CMe. BTI-CMe was properly transcribed and translated as indicated by northern and western blot, with a level of expression in transgenic wheat seeds up to 1.1% of total extracted protein. No expression was detected in untransformed wheat seeds. Functional integrity of BTI-CMe was confirmed by trypsin inhibitor activity assay. The significant reduction of the survival rate of the Angoumois grain moth (Sitotroga cerealella, Lepidoptera: Gelechiidae), reared on transgenic wheat seeds expressing the trypsin inhibitor BTI-CMe, compared to the untransformed control confirmed the potential of BTI-CMe for the increase of insect resistance. However, only early-instar larvae were inhibited in transgenic seeds and expression of BTI-CMe protein in transgenic leaves did not have a significant protective effect against leaf-feeding insects. date_type: published date: 1999-01 publication: Molecular Breeding volume: 5 number: 1 pagerange: 56-63 id_number: 10.1023/A:10096599 institution: Agronomica department: Biotecnologia_2014 refereed: TRUE issn: 1380-3743 official_url: https://link.springer.com/article/10.1023/A:1009659911798 comprojects_type: MINECO comprojects_code: BIO96-2303 comprojects_leader: Pilar Carbonero Zalduegui comprojects_title: Identificación y mutagenización de genes de cereales para su utilización transgénica frente a plagas de insectos citation: Altpeter, Fredy and Diaz Rodriguez, Isabel and McAuslane, Heather and Gaddour, Kamel and Carbonero Zalduegui, Pilar and Vasil, Indra K. (1999). Increased insect resistance in transgenic wheat stably expressing trypsin inhibitor CMe. "Molecular Breeding", v. 5 (n. 1); pp. 56-63. ISSN 1380-3743. https://doi.org/10.1023/A:10096599 . document_url: https://oa.upm.es/53944/1/Molecular_Breeding_1999.pdf