TY  - JOUR
VL  - 70
Y1  - 2019/03//
SP  - 2143
TI  - Repression of drought-induced cysteine-protease genes alters barley leaf structure and responses to abiotic and biotic stresses
AV  - public
N2  - To survive under water deficiency, plants alter gene expression patterns, make structural and physiological adjustments, and optimize the use of water. Rapid degradation and turnover of proteins is required for effective nutrient recycling. Here, we examined the transcriptional responses of the C1A cysteine protease family to drought in barley and found that four genes were up-regulated in stressed plants. Knock-down lines for the protease-encoding genes HvPap-1 and HvPap-19 showed unexpected changes in leaf cuticle thickness and stomatal pore area. The efficiency of photosystem II and the total amount of proteins were almost unaltered in stressed transgenic plants while both parameters decreased in stressed wild-type plants. Although the patterns of proteolytic activities in the knock-down lines did not change, the amino acid accumulation increased in response to drought, concomitant with a higher ABA content. Whilst jasmonic acid (JA) and JA-Ile concentrations increased in stressed leaves of the wild-type and the HvPap-1 knock-down lines, their levels were lower in the HvPap-19 knock-down lines, suggesting the involvement of a specific hormone interaction in the process. Our data indicate that the changes in leaf cuticle thickness and stomatal pore area had advantageous effects on leaf defense against fungal infection and mite feeding mediated by Magnaporthe oryzae and Tetranychus urticae, respectively.
KW  - Abiotic/biotic stress; cysteine-protease; drought; leaf cuticles; stomata; water deprivation
SN  - 0022-0957
ID  - upm63297
UR  - https://doi.org/10.1093/jxb/ery410
A1  - Gómez Sánchez, Andrea
A1  - González Melendi, Pablo
A1  - Santamaría Fernández, María Estrella
A1  - Arbona Mengual, Vicente
A1  - López Gonzálvez, Ángeles
A1  - García, Antonia
A1  - Hensel, Goetz
A1  - Kumlehn, Jochen
A1  - Martinez Muñoz, Manuel
A1  - Diaz Rodriguez, Isabel
IS  - 7
JF  - Journal of Experimental Botany
EP  - 2155
PB  - Oxford University Press
ER  -