eprintid: 63297 rev_number: 15 eprint_status: archive userid: 1903 dir: disk0/00/06/32/97 datestamp: 2020-12-10 11:15:12 lastmod: 2021-03-03 08:11:13 status_changed: 2021-03-03 08:11:13 type: article metadata_visibility: show creators_name: Gómez Sánchez, Andrea creators_name: González Melendi, Pablo creators_name: Santamaría Fernández, María Estrella creators_name: Arbona Mengual, Vicente creators_name: López Gonzálvez, Ángeles creators_name: García, Antonia creators_name: Hensel, Goetz creators_name: Kumlehn, Jochen creators_name: Martinez Muñoz, Manuel creators_name: Diaz Rodriguez, Isabel creators_id: pablo.melendi@upm.es creators_id: m.martinez@upm.es creators_id: i.diaz@upm.es title: Repression of drought-induced cysteine-protease genes alters barley leaf structure and responses to abiotic and biotic stresses ispublished: pub subjects: biologia keywords: Abiotic/biotic stress; cysteine-protease; drought; leaf cuticles; stomata; water deprivation abstract: To survive under water deficiency, plants alter gene expression patterns, make structural and physiological adjustments, and optimize the use of water. Rapid degradation and turnover of proteins is required for effective nutrient recycling. Here, we examined the transcriptional responses of the C1A cysteine protease family to drought in barley and found that four genes were up-regulated in stressed plants. Knock-down lines for the protease-encoding genes HvPap-1 and HvPap-19 showed unexpected changes in leaf cuticle thickness and stomatal pore area. The efficiency of photosystem II and the total amount of proteins were almost unaltered in stressed transgenic plants while both parameters decreased in stressed wild-type plants. Although the patterns of proteolytic activities in the knock-down lines did not change, the amino acid accumulation increased in response to drought, concomitant with a higher ABA content. Whilst jasmonic acid (JA) and JA-Ile concentrations increased in stressed leaves of the wild-type and the HvPap-1 knock-down lines, their levels were lower in the HvPap-19 knock-down lines, suggesting the involvement of a specific hormone interaction in the process. Our data indicate that the changes in leaf cuticle thickness and stomatal pore area had advantageous effects on leaf defense against fungal infection and mite feeding mediated by Magnaporthe oryzae and Tetranychus urticae, respectively. date: 2019-03 date_type: published publisher: Oxford University Press official_url: https://doi.org/10.1093/jxb/ery410 id_number: 10.1093/jxb/ery410 full_text_status: public publication: Journal of Experimental Botany volume: 70 number: 7 pagerange: 2143-2155 institution: Biotecnologia_Genomica department: otro refereed: TRUE issn: 0022-0957 rights: by-nc-nd comprojects_type: MINECO comprojects_code: BIO2104-53508-R comprojects_title: Senescencia foliar en cebada: asociación entre la degradación-movilización de proteínas inducida por estrés abiótico/biótico y la calidad del grano citation: Gómez Sánchez, Andrea and González Melendi, Pablo and Santamaría Fernández, María Estrella and Arbona Mengual, Vicente and López Gonzálvez, Ángeles and García, Antonia and Hensel, Goetz and Kumlehn, Jochen and Martinez Muñoz, Manuel and Diaz Rodriguez, Isabel (2019). Repression of drought-induced cysteine-protease genes alters barley leaf structure and responses to abiotic and biotic stresses. "Journal of Experimental Botany", v. 70 (n. 7); pp. 2143-2155. ISSN 0022-0957. https://doi.org/10.1093/jxb/ery410 . document_url: https://oa.upm.es/63297/1/INVE_MEM_2019_309586.pdf