Differential in vitro and in vivo effect of barley cysteine and serine protease inhibitors on phytopathogenic microorganisms

Carrillo Gil, Laura; Herrero, Ignacio; Cambra Marin, Ines; Sánchez-Monge Laguna de Rins, Rosa; Diaz Rodriguez, Isabel y Martinez Muñoz, Manuel (2011). Differential in vitro and in vivo effect of barley cysteine and serine protease inhibitors on phytopathogenic microorganisms. "Plant Physiology and Biochemistry", v. 49 (n. 10); pp. 1191-1200. ISSN 0981-9428. https://doi.org/10.1016/j.plaphy.2011.03.012.

Descripción

Título: Differential in vitro and in vivo effect of barley cysteine and serine protease inhibitors on phytopathogenic microorganisms
Autor/es:
  • Carrillo Gil, Laura
  • Herrero, Ignacio
  • Cambra Marin, Ines
  • Sánchez-Monge Laguna de Rins, Rosa
  • Diaz Rodriguez, Isabel
  • Martinez Muñoz, Manuel
Tipo de Documento: Artículo
Título de Revista/Publicación: Plant Physiology and Biochemistry
Fecha: Octubre 2011
Volumen: 49
Materias:
Escuela: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Departamento: Biotecnologia [hasta 2014]
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

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Resumen

Protease inhibitors from plants have been involved in defence mechanisms against pests and pathogens. Phytocystatins and trypsin/α-amylase inhibitors are two of the best characterized protease inhibitor families in plants. In barley, thirteen cystatins (HvCPI-1 to 13) and the BTI-CMe trypsin inhibitor have been previously studied. Their capacity to inhibit pest digestive proteases, and the negative in vivo effect caused by plants expressing these inhibitors on pests support the defence function of these proteins. Barley cystatins are also able to inhibit in vitro fungal growth. However, the antifungal effect of these inhibitors in vivo had not been previously tested. Moreover, their in vitro and in vivo effect on plant pathogenous bacteria is still unknown. In order to obtain new insights on this feature, in vitro assays were made against different bacterial and fungal pathogens of plants using the trypsin inhibitor BTI-CMe and the thirteen barley cystatins. Most barley cystatins and the BTI-CMe inhibitor were able to inhibit mycelial growth but no bacterial growth. Transgenic Arabidopsis plants independently expressing the BTI-CMe inhibitor and the cystatin HvCPI-6 were tested against the same bacterial and fungal pathogens. Neither the HvCPI-6 expressing transgenic plants nor the BTI-CMe ones were more resistant to plant pathogen fungi and bacteria than control Arabidopsis plants. The differences observed between the in vitro and in planta assays against phytopathogenic fungi are discussed

Más información

ID de Registro: 11220
Identificador DC: http://oa.upm.es/11220/
Identificador OAI: oai:oa.upm.es:11220
Identificador DOI: 10.1016/j.plaphy.2011.03.012
Depositado por: Memoria Investigacion
Depositado el: 09 Jul 2012 08:07
Ultima Modificación: 20 Abr 2016 19:22
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