Differential in vitro and in vivo effect of barley cysteine and serine protease inhibitors on phytopathogenic microorganisms

Carrillo Gil, Laura and Herrero, Ignacio and Cambra Marin, Ines and Sánchez-Monge Laguna de Rins, Rosa and Diaz Rodriguez, Isabel and Martinez Muñoz, Manuel (2011). Differential in vitro and in vivo effect of barley cysteine and serine protease inhibitors on phytopathogenic microorganisms. "Plant Physiology and Biochemistry", v. 49 (n. 10); pp. 1191-1200. ISSN 0981-9428. https://doi.org/10.1016/j.plaphy.2011.03.012.

Description

Title: Differential in vitro and in vivo effect of barley cysteine and serine protease inhibitors on phytopathogenic microorganisms
Author/s:
  • Carrillo Gil, Laura
  • Herrero, Ignacio
  • Cambra Marin, Ines
  • Sánchez-Monge Laguna de Rins, Rosa
  • Diaz Rodriguez, Isabel
  • Martinez Muñoz, Manuel
Item Type: Article
Título de Revista/Publicación: Plant Physiology and Biochemistry
Date: October 2011
ISSN: 0981-9428
Volume: 49
Subjects:
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Protease inhibitors from plants have been involved in defence mechanisms against pests and pathogens. Phytocystatins and trypsin/α-amylase inhibitors are two of the best characterized protease inhibitor families in plants. In barley, thirteen cystatins (HvCPI-1 to 13) and the BTI-CMe trypsin inhibitor have been previously studied. Their capacity to inhibit pest digestive proteases, and the negative in vivo effect caused by plants expressing these inhibitors on pests support the defence function of these proteins. Barley cystatins are also able to inhibit in vitro fungal growth. However, the antifungal effect of these inhibitors in vivo had not been previously tested. Moreover, their in vitro and in vivo effect on plant pathogenous bacteria is still unknown. In order to obtain new insights on this feature, in vitro assays were made against different bacterial and fungal pathogens of plants using the trypsin inhibitor BTI-CMe and the thirteen barley cystatins. Most barley cystatins and the BTI-CMe inhibitor were able to inhibit mycelial growth but no bacterial growth. Transgenic Arabidopsis plants independently expressing the BTI-CMe inhibitor and the cystatin HvCPI-6 were tested against the same bacterial and fungal pathogens. Neither the HvCPI-6 expressing transgenic plants nor the BTI-CMe ones were more resistant to plant pathogen fungi and bacteria than control Arabidopsis plants. The differences observed between the in vitro and in planta assays against phytopathogenic fungi are discussed

More information

Item ID: 11220
DC Identifier: http://oa.upm.es/11220/
OAI Identifier: oai:oa.upm.es:11220
DOI: 10.1016/j.plaphy.2011.03.012
Deposited by: Memoria Investigacion
Deposited on: 09 Jul 2012 08:07
Last Modified: 20 Apr 2016 19:22
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