Identification of a homolog of Arabidopsis DSP4 (SEX4) in chestnut: its induction and accumulation in stem amyloplasts during winter or in response to the cold_

Berrocal Lobo, Marta and Ibáñez, Cristian and Acebo Pais, Paloma and Ramos Aranguren, Alberto and Pérez Solís, Estefanía and Collada Collada, Maria Carmen and Casado García, Rosa and Aragoncillo Ballesteros, Cipriano and Allona Alberich, Isabel Marta (2011). Identification of a homolog of Arabidopsis DSP4 (SEX4) in chestnut: its induction and accumulation in stem amyloplasts during winter or in response to the cold_. "Plant, Cell & Environment", v. 34 (n. 10); pp. 1693-1704. ISSN 0140-7791. https://doi.org/10.1111/j.1365-3040.2011.02365.x.

Description

Title: Identification of a homolog of Arabidopsis DSP4 (SEX4) in chestnut: its induction and accumulation in stem amyloplasts during winter or in response to the cold_
Author/s:
  • Berrocal Lobo, Marta
  • Ibáñez, Cristian
  • Acebo Pais, Paloma
  • Ramos Aranguren, Alberto
  • Pérez Solís, Estefanía
  • Collada Collada, Maria Carmen
  • Casado García, Rosa
  • Aragoncillo Ballesteros, Cipriano
  • Allona Alberich, Isabel Marta
Item Type: Article
Título de Revista/Publicación: Plant, Cell & Environment
Date: October 2011
ISSN: 0140-7791
Volume: 34
Subjects:
Faculty: E.T.S.I. Montes (UPM)
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Oligosaccharide synthesis is an important cryoprotection strategy used by woody plants during winter dormancy. At the onset of autumn, starch stored in the stem and buds is broken down in response to the shorter days and lower temperatures resulting in the buildup of oligosaccharides. Given that the enzyme DSP4 is necessary for diurnal starch degradation in Arabidopsis leaves, this study was designed to address the role of DSP4 in this seasonal process in Castanea sativa Mill. The expression pattern of the CsDSP4 gene in cells of the chestnut stem was found to parallel starch catabolism. In this organ, DSP4 protein levels started to rise at the start of autumn and elevated levels persisted until the onset of spring. In addition, exposure of chestnut plantlets to 4 °C induced the expression of the CsDSP4 gene. In dormant trees or cold-stressed plantlets, the CsDSP4 protein was immunolocalized both in the amyloplast stroma and nucleus of stem cells, whereas in the conditions of vegetative growth, immunofluorescence was only detected in the nucleus. The studies indicate a potential role for DSP4 in starch degradation and cold acclimation following low temperature exposure during activity–dormancy transition.

More information

Item ID: 11510
DC Identifier: http://oa.upm.es/11510/
OAI Identifier: oai:oa.upm.es:11510
DOI: 10.1111/j.1365-3040.2011.02365.x
Official URL: http://onlinelibrary.wiley.com/doi/10.1111/j.1365-3040.2011.02365.x/suppinfo
Deposited by: Memoria Investigacion
Deposited on: 19 Jul 2012 11:15
Last Modified: 13 May 2015 13:00
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