Identification of a homolog of Arabidopsis DSP4 (SEX4) in chestnut: its induction and accumulation in stem amyloplasts during winter or in response to the cold_

Berrocal Lobo, Marta; Ibáñez, Cristian; Acebo Pais, Paloma; Ramos Aranguren, Alberto; Pérez Solís, Estefanía; Collada Collada, Maria Carmen; Casado García, Rosa; Aragoncillo Ballesteros, Cipriano y Allona Alberich, Isabel Marta (2011). Identification of a homolog of Arabidopsis DSP4 (SEX4) in chestnut: its induction and accumulation in stem amyloplasts during winter or in response to the cold_. "Plant, Cell & Environment", v. 34 (n. 10); pp. 1693-1704. ISSN 0140-7791. https://doi.org/10.1111/j.1365-3040.2011.02365.x.

Descripción

Título: Identification of a homolog of Arabidopsis DSP4 (SEX4) in chestnut: its induction and accumulation in stem amyloplasts during winter or in response to the cold_
Autor/es:
  • Berrocal Lobo, Marta
  • Ibáñez, Cristian
  • Acebo Pais, Paloma
  • Ramos Aranguren, Alberto
  • Pérez Solís, Estefanía
  • Collada Collada, Maria Carmen
  • Casado García, Rosa
  • Aragoncillo Ballesteros, Cipriano
  • Allona Alberich, Isabel Marta
Tipo de Documento: Artículo
Título de Revista/Publicación: Plant, Cell & Environment
Fecha: Octubre 2011
Volumen: 34
Materias:
Escuela: E.T.S.I. Montes (UPM) [antigua denominación]
Departamento: Biotecnologia [hasta 2014]
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

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Resumen

Oligosaccharide synthesis is an important cryoprotection strategy used by woody plants during winter dormancy. At the onset of autumn, starch stored in the stem and buds is broken down in response to the shorter days and lower temperatures resulting in the buildup of oligosaccharides. Given that the enzyme DSP4 is necessary for diurnal starch degradation in Arabidopsis leaves, this study was designed to address the role of DSP4 in this seasonal process in Castanea sativa Mill. The expression pattern of the CsDSP4 gene in cells of the chestnut stem was found to parallel starch catabolism. In this organ, DSP4 protein levels started to rise at the start of autumn and elevated levels persisted until the onset of spring. In addition, exposure of chestnut plantlets to 4 °C induced the expression of the CsDSP4 gene. In dormant trees or cold-stressed plantlets, the CsDSP4 protein was immunolocalized both in the amyloplast stroma and nucleus of stem cells, whereas in the conditions of vegetative growth, immunofluorescence was only detected in the nucleus. The studies indicate a potential role for DSP4 in starch degradation and cold acclimation following low temperature exposure during activity–dormancy transition.

Más información

ID de Registro: 11510
Identificador DC: http://oa.upm.es/11510/
Identificador OAI: oai:oa.upm.es:11510
Identificador DOI: 10.1111/j.1365-3040.2011.02365.x
URL Oficial: http://onlinelibrary.wiley.com/doi/10.1111/j.1365-3040.2011.02365.x/suppinfo
Depositado por: Memoria Investigacion
Depositado el: 19 Jul 2012 11:15
Ultima Modificación: 13 May 2015 13:00
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