Optimization of a label-free biosensor vertically characterized based on a periodic lattice of high aspect ratio SU-8 nano-pillars with a simplified 2D theoretical model

Casquel del Campo, Rafael; Holgado Bolaños, Miguel; Sanza Gutiérrez, Francisco Javier; Laguna Heras, Maria Fe; Angulo Barrios, Carlos; López-Romero Moraleda, David; Ortega, Francisco J.; Bañuls, María José; Puchades, Rosa y Maquieira, A. (2011). Optimization of a label-free biosensor vertically characterized based on a periodic lattice of high aspect ratio SU-8 nano-pillars with a simplified 2D theoretical model. "Physica Status Solid c", v. 8 (n. 3); pp. 1087-1092. ISSN 1610-1642. https://doi.org/10.1002/pssc.201000412.

Descripción

Título: Optimization of a label-free biosensor vertically characterized based on a periodic lattice of high aspect ratio SU-8 nano-pillars with a simplified 2D theoretical model
Autor/es:
  • Casquel del Campo, Rafael
  • Holgado Bolaños, Miguel
  • Sanza Gutiérrez, Francisco Javier
  • Laguna Heras, Maria Fe
  • Angulo Barrios, Carlos
  • López-Romero Moraleda, David
  • Ortega, Francisco J.
  • Bañuls, María José
  • Puchades, Rosa
  • Maquieira, A.
Tipo de Documento: Artículo
Título de Revista/Publicación: Physica Status Solid c
Fecha: 2011
Volumen: 8
Materias:
Palabras Clave Informales: label free biosensor, optimization, simplified calculation
Escuela: E.T.S.I. Industriales (UPM)
Departamento: Física Aplicada a la Ingeniería Industrial [hasta 2014]
Licencias Creative Commons: Reconocimiento - Sin obra derivada - No comercial

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Resumen

We have recently demonstrated a biosensor based on a lattice of SU8 pillars on a 1 μm SiO2/Si wafer by measuring vertically reflectivity as a function of wavelength. The biodetection has been proven with the combination of Bovine Serum Albumin (BSA) protein and its antibody (antiBSA). A BSA layer is attached to the pillars; the biorecognition of antiBSA involves a shift in the reflectivity curve, related with the concentration of antiBSA. A detection limit in the order of 2 ng/ml is achieved for a rhombic lattice of pillars with a lattice parameter (a) of 800 nm, a height (h) of 420 nm and a diameter(d) of 200 nm. These results correlate with calculations using 3D-finite difference time domain method. A 2D simplified model is proposed, consisting of a multilayer model where the pillars are turned into a 420 nm layer with an effective refractive index obtained by using Beam Propagation Method (BPM) algorithm. Results provided by this model are in good correlation with experimental data, reaching a reduction in time from one day to 15 minutes, giving a fast but accurate tool to optimize the design and maximizing sensitivity, and allows analyzing the influence of different variables (diameter, height and lattice parameter). Sensitivity is obtained for a variety of configurations, reaching a limit of detection under 1 ng/ml. Optimum design is not only chosen because of its sensitivity but also its feasibility, both from fabrication (limited by aspect ratio and proximity of the pillars) and fluidic point of view. (© 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

Más información

ID de Registro: 11875
Identificador DC: http://oa.upm.es/11875/
Identificador OAI: oai:oa.upm.es:11875
Identificador DOI: 10.1002/pssc.201000412
URL Oficial: http://www.interscience.wiley.com/
Depositado por: Memoria Investigacion
Depositado el: 07 Mar 2013 08:17
Ultima Modificación: 28 Jun 2016 07:00
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