Optimization of a label-free biosensor vertically characterized based on a periodic lattice of high aspect ratio SU-8 nano-pillars with a simplified 2D theoretical model

Casquel del Campo, Rafael and Holgado Bolaños, Miguel and Sanza Gutiérrez, Francisco Javier and Laguna Heras, Maria Fe and Angulo Barrios, Carlos and López-Romero Moraleda, David and Ortega, Francisco J. and Bañuls, María José and Puchades, Rosa and Maquieira, A. (2011). Optimization of a label-free biosensor vertically characterized based on a periodic lattice of high aspect ratio SU-8 nano-pillars with a simplified 2D theoretical model. "Physica Status Solid c", v. 8 (n. 3); pp. 1087-1092. ISSN 1610-1642. https://doi.org/10.1002/pssc.201000412.

Description

Title: Optimization of a label-free biosensor vertically characterized based on a periodic lattice of high aspect ratio SU-8 nano-pillars with a simplified 2D theoretical model
Author/s:
  • Casquel del Campo, Rafael
  • Holgado Bolaños, Miguel
  • Sanza Gutiérrez, Francisco Javier
  • Laguna Heras, Maria Fe
  • Angulo Barrios, Carlos
  • López-Romero Moraleda, David
  • Ortega, Francisco J.
  • Bañuls, María José
  • Puchades, Rosa
  • Maquieira, A.
Item Type: Article
Título de Revista/Publicación: Physica Status Solid c
Date: 2011
ISSN: 1610-1642
Volume: 8
Subjects:
Freetext Keywords: label free biosensor, optimization, simplified calculation
Faculty: E.T.S.I. Industriales (UPM)
Department: Física Aplicada a la Ingeniería Industrial [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

We have recently demonstrated a biosensor based on a lattice of SU8 pillars on a 1 μm SiO2/Si wafer by measuring vertically reflectivity as a function of wavelength. The biodetection has been proven with the combination of Bovine Serum Albumin (BSA) protein and its antibody (antiBSA). A BSA layer is attached to the pillars; the biorecognition of antiBSA involves a shift in the reflectivity curve, related with the concentration of antiBSA. A detection limit in the order of 2 ng/ml is achieved for a rhombic lattice of pillars with a lattice parameter (a) of 800 nm, a height (h) of 420 nm and a diameter(d) of 200 nm. These results correlate with calculations using 3D-finite difference time domain method. A 2D simplified model is proposed, consisting of a multilayer model where the pillars are turned into a 420 nm layer with an effective refractive index obtained by using Beam Propagation Method (BPM) algorithm. Results provided by this model are in good correlation with experimental data, reaching a reduction in time from one day to 15 minutes, giving a fast but accurate tool to optimize the design and maximizing sensitivity, and allows analyzing the influence of different variables (diameter, height and lattice parameter). Sensitivity is obtained for a variety of configurations, reaching a limit of detection under 1 ng/ml. Optimum design is not only chosen because of its sensitivity but also its feasibility, both from fabrication (limited by aspect ratio and proximity of the pillars) and fluidic point of view. (© 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

More information

Item ID: 11875
DC Identifier: http://oa.upm.es/11875/
OAI Identifier: oai:oa.upm.es:11875
DOI: 10.1002/pssc.201000412
Official URL: http://www.interscience.wiley.com/
Deposited by: Memoria Investigacion
Deposited on: 07 Mar 2013 08:17
Last Modified: 28 Jun 2016 07:00
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