Cita
Samol, Iga; Rossig, Claudia; Buhr, Frank; Springer, Armin; Pollmann, Stephan; Lahroussi, Abder; von Wettstein, Diter; Reinbothe, Christiane y Reinbothe, Steffen
(2011).
The Outer Chloroplast Envelope Protein OEP16-1 for Plastid Import of NADPH:Protochlorophyllide Oxidoreductase A in Arabidopsis thaliana.
"Plant And Cell Physiology", v. 52
(n. 1);
pp. 96-111.
ISSN 0032-0781.
https://doi.org/10.1093/pcp/pcq177.
Resumen
The outer plastid envelope protein OEP16-1 was previously identified as an amino acid-selective channel protein and translocation pore for NADPH:protochlorophyllide oxidoreductase A (PORA). Reverse genetic approaches used to dissect these mutually not exclusive functions of OEP16-1 in planta have led to descriptions of different phenotypes resulting from the presence of several mutant lines in the SALK_024018 seed stock. In addition to the T-DNA insertion in the AtOEP16-1 gene, lines were purified that contain two additional T-DNA insertions and as yet unidentified point mutations. In a first attempt to resolve the genetic basis of four different lines in the SALK_024018 seed stock, we used genetic transformation with the OEP16-1 cDNA and segregation analyses after crossing out presumed point mutations. We show that AtOEP16-1 is involved in PORA precursor import and by virtue of this activity confers photoprotection onto etiolated seedlings during greening
