Endosymbiotic bacteria nodulating a new endemic lupine Lupinus mariae-josephi from alkaline soils in Eastern Spain represent a new lineage within the Bradyrhizobium genus

Sanchez Cañizares, Carmen and Rey Navarro, Luis and Ruiz Argüeso, Tomas-Andres and Imperial Ródenas, Juan and Duran Wendt, David Ricardo (2011). Endosymbiotic bacteria nodulating a new endemic lupine Lupinus mariae-josephi from alkaline soils in Eastern Spain represent a new lineage within the Bradyrhizobium genus. "Systematic And Applied Microbiology", v. 34 (n. 3); pp. 207-215. ISSN 0723-2020. https://doi.org/10.1016/j.syapm.2010.11.020.

Description

Title: Endosymbiotic bacteria nodulating a new endemic lupine Lupinus mariae-josephi from alkaline soils in Eastern Spain represent a new lineage within the Bradyrhizobium genus
Author/s:
  • Sanchez Cañizares, Carmen
  • Rey Navarro, Luis
  • Ruiz Argüeso, Tomas-Andres
  • Imperial Ródenas, Juan
  • Duran Wendt, David Ricardo
Item Type: Article
Título de Revista/Publicación: Systematic And Applied Microbiology
Date: May 2011
ISSN: 0723-2020
Volume: 34
Subjects:
Faculty: E.T.S.I. Agrónomos (UPM) [antigua denominación]
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Lupinus mariae-josephi is a recently described endemic Lupinus species from a small area in Eastern Spain where it thrives in soils with active lime and high pH. The L. mariae-josephi root symbionts were shown to be very slow-growing bacteria with different phenotypic and symbiotic characteristics from those of Bradyrhizobium strains nodulating other Lupinus. Their phylogenetic status was examined by multilocus sequence analyses of four housekeeping genes (16S rRNA, glnII, recA, and atpD) and showed the existence of a distinct evolutionary lineage for L. mariae-josephi that also included Bradyrhizobium jicamae. Within this lineage, the tested isolates clustered in three different sub-groups that might correspond to novel sister Bradyrhizobium species. These core gene analyses consistently showed that all the endosymbiotic bacteria isolated from other Lupinus species of the Iberian Peninsula were related to strains of the B. canariense or B. japonicum lineages and were separate from the L. mariae-josephi isolates. Phylogenetic analysis based on nodC symbiotic gene sequences showed that L. mariae-josephi bacteria also constituted a new symbiotic lineage distant from those previously defined in the genus Bradyrhizobium. In contrast, the nodC genes of isolates from other Lupinus spp. from the Iberian Peninsula were again clearly related to the B. canariense and B. japonicum bv. genistearum lineages. Speciation of L. mariae-josephi bradyrhizobia may result from the colonization of a singular habitat by their unique legume host.

More information

Item ID: 12390
DC Identifier: http://oa.upm.es/12390/
OAI Identifier: oai:oa.upm.es:12390
DOI: 10.1016/j.syapm.2010.11.020
Deposited by: Memoria Investigacion
Deposited on: 10 Aug 2012 09:17
Last Modified: 21 Apr 2016 11:38
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