Development of an electrochemical maltose biosensor

Moron Fernandez, Carlos and Tremps Guerra, Enrique and Garcia Garcia, Alfonso and Somolinos Sanchez, Jose Andres (2012). Development of an electrochemical maltose biosensor. In: "1st International Conference on Materials and Applications for Sensors and Transducers (IC-MAST)", 13/05/2011 - 17/05/2011, Isla Kos, Grecia. ISBN 978-3-03785-292-7. pp. 131-132.

Description

Title: Development of an electrochemical maltose biosensor
Author/s:
  • Moron Fernandez, Carlos
  • Tremps Guerra, Enrique
  • Garcia Garcia, Alfonso
  • Somolinos Sanchez, Jose Andres
Item Type: Presentation at Congress or Conference (Article)
Event Title: 1st International Conference on Materials and Applications for Sensors and Transducers (IC-MAST)
Event Dates: 13/05/2011 - 17/05/2011
Event Location: Isla Kos, Grecia
Title of Book: Materials and Applications for Sensors and Transducers
Date: 2012
ISBN: 978-3-03785-292-7
Subjects:
Faculty: E.U. de Arquitectura Técnica (UPM)
Department: Tecnología de la Edificación
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

In this work, electrochemical maltose biosensors based on mutants of the maltose binding protein (MBP) are developed. A ruthenium II complex (Ru II ), which is covalently attached to MBP, serves as an electrochemical reporter of MBP conformational changes. Biosensors were made through direct attachment of Ru II complex modified MBP to gold electrode surfaces. The responses of some individual mutants were evaluated using square wave voltammetry. A maltose-dependent change in Faradic current and capacitance was observed. It is therefore demonstrated that biosensors using generically this family of bacterial periplasmic binding proteins (bPBP) can be made lending themselves to facile biorecognition element preparation and low cost electrochemical transduction.

More information

Item ID: 13453
DC Identifier: http://oa.upm.es/13453/
OAI Identifier: oai:oa.upm.es:13453
Official URL: http://www.ttp.net/978-3-03785-292-7.html
Deposited by: Memoria Investigacion
Deposited on: 18 Oct 2012 10:28
Last Modified: 21 Apr 2016 12:46
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