Differential Micro RNA Expression in PBMC from Multiple Sclerosis Patients

Otaegui, David and Baranzini, Sergio E. and Armañanzas Arnedillo, Ruben and Calvo, Borja and Muñoz Culla, Maider and Khankhanian, Puya and Inza Cano, Iñaki and Lozano, Jose Antonio and Asensio, Ana and Olaskoaga, Javier and López de Munain, Adolfo (2009). Differential Micro RNA Expression in PBMC from Multiple Sclerosis Patients. "Plos One", v. 4 (n. 7); pp. 1-9. ISSN 1932-6203. https://doi.org/10.1371/journal.pone.0006309.

Description

Title: Differential Micro RNA Expression in PBMC from Multiple Sclerosis Patients
Author/s:
  • Otaegui, David
  • Baranzini, Sergio E.
  • Armañanzas Arnedillo, Ruben
  • Calvo, Borja
  • Muñoz Culla, Maider
  • Khankhanian, Puya
  • Inza Cano, Iñaki
  • Lozano, Jose Antonio
  • Asensio, Ana
  • Olaskoaga, Javier
  • López de Munain, Adolfo
Item Type: Article
Título de Revista/Publicación: Plos One
Date: 2009
ISSN: 1932-6203
Volume: 4
Subjects:
Faculty: Facultad de Informática (UPM)
Department: Otro
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Differences in gene expression patterns have been documented not only in Multiple Sclerosis patients versus healthy controls but also in the relapse of the disease. Recently a new gene expression modulator has been identified: the microRNA or miRNA. The aim of this work is to analyze the possible role of miRNAs in multiple sclerosis, focusing on the relapse stage. We have analyzed the expression patterns of 364 miRNAs in PBMC obtained from multiple sclerosis patients in relapse status, in remission status and healthy controls. The expression patterns of the miRNAs with significantly different expression were validated in an independent set of samples. In order to determine the effect of the miRNAs, the expression of some predicted target genes of these were studied by qPCR. Gene interaction networks were constructed in order to obtain a co-expression and multivariate view of the experimental data. The data analysis and later validation reveal that two miRNAs (hsa-miR-18b and hsa-miR-599) may be relevant at the time of relapse and that another miRNA (hsa-miR-96) may be involved in remission. The genes targeted by hsa-miR-96 are involved in immunological pathways as Interleukin signaling and in other pathways as wnt signaling. This work highlights the importance of miRNA expression in the molecular mechanisms implicated in the disease. Moreover, the proposed involvement of these small molecules in multiple sclerosis opens up a new therapeutic approach to explore and highlight some candidate biomarker targets in MS

More information

Item ID: 13938
DC Identifier: http://oa.upm.es/13938/
OAI Identifier: oai:oa.upm.es:13938
DOI: 10.1371/journal.pone.0006309
Official URL: http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0006309
Deposited by: Memoria Investigacion
Deposited on: 21 Dec 2012 11:34
Last Modified: 21 Apr 2016 13:22
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