The Agrobacterium vitis T-6b oncoprotein induces auxin-independent cell expansion in tobacco

Clément, Bernadette and Pollmann, Stephan and Weiler, Elmar W. and Urbanczyk Wochniak, Ewa and Otten, León (2006). The Agrobacterium vitis T-6b oncoprotein induces auxin-independent cell expansion in tobacco. "Plant Journal", v. 45 ; pp. 1017-1027. ISSN 0960-7412.

Description

Title: The Agrobacterium vitis T-6b oncoprotein induces auxin-independent cell expansion in tobacco
Author/s:
  • Clément, Bernadette
  • Pollmann, Stephan
  • Weiler, Elmar W.
  • Urbanczyk Wochniak, Ewa
  • Otten, León
Item Type: Article
Título de Revista/Publicación: Plant Journal
Date: 2006
ISSN: 0960-7412
Volume: 45
Subjects:
Freetext Keywords: Agrobacterium, metabolite profiling, oncogenes, osmolytes, plast family, tumors.
Faculty: Centro de Investigación en Biotecnología y Genómica de Plantas (CBGP) (UPM)
Department: Biotecnologia [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Among the Agrobacterium T-DNA genes, rolB, rolC, orf13, orf8, lso, 6b and several other genes encode weakly homologous proteins with remarkable effects on plant growth. The 6b oncogene induces tumors and enations. In order to study its properties we have used transgenic tobacco plants that carry a dexamethasone-inducible 6b gene, dex-T-6b. Upon induction, dex-T-6b plants develop a large array of morphological modifications, some of which involve abnormal cell expansion. In the present investigation, dex-T-6b-induced expansion was studied in intact leaves and an in vitro leaf disc system. Although T-6b and indole-3-acetic acid (IAA) both induced expansion and were non-additive, T-6b expression did not increase IAA levels, nor did it induce an IAA-responsive gene. Fusicoccin (FC) is known to stimulate expansion by increasing cell wall plasticity. T-6b- and FC-induced expansion were additive at saturating FC concentrations, indicating that T-6b does not act by a similar mechanism to FC. T-6b expression led to higher leaf osmolality values, in contrast to FC, suggesting that the T-6b gene induces expansion by increasing osmolyte concentrations. Metabolite profiling showed that glucose and fructose played a major role in this increase. We infer that T-6b disrupts the osmoregulatory controls that govern cell expansion during development and wound healing.

More information

Item ID: 14045
DC Identifier: http://oa.upm.es/14045/
OAI Identifier: oai:oa.upm.es:14045
Official URL: http://ibmp.u-strasbg.fr/
Deposited by: Memoria Investigacion
Deposited on: 20 Dec 2012 11:48
Last Modified: 21 Apr 2016 13:31
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