A bacterial cysteine protease effector protein interferes with photosynthesis to suppress plant innate immune responses

Rodríguez Herva, Jose Juan and Gonzalez-Melendi de Leon, Pablo and Cuartas-Lanza, Raquel and Antúnez-Lamas, María and Río-Álvarez, Isabel and Li, Ziduo and López-Torrejón, Gema and Diaz Rodriguez, Isabel and Pozo, Juan C. del and Chakravarthy, Suma and Collmer, Alan and Rodriguez Palenzuela, Pablo and Lopez Solanilla, Emilia (2012). A bacterial cysteine protease effector protein interferes with photosynthesis to suppress plant innate immune responses. "Cellular Microbiology", v. 14 (n. 5); pp. 669-681. ISSN 1462-5814. https://doi.org/10.1111/j.1462-5822.2012.01749.x.

Description

Title: A bacterial cysteine protease effector protein interferes with photosynthesis to suppress plant innate immune responses
Author/s:
  • Rodríguez Herva, Jose Juan
  • Gonzalez-Melendi de Leon, Pablo
  • Cuartas-Lanza, Raquel
  • Antúnez-Lamas, María
  • Río-Álvarez, Isabel
  • Li, Ziduo
  • López-Torrejón, Gema
  • Diaz Rodriguez, Isabel
  • Pozo, Juan C. del
  • Chakravarthy, Suma
  • Collmer, Alan
  • Rodriguez Palenzuela, Pablo
  • Lopez Solanilla, Emilia
Item Type: Article
Título de Revista/Publicación: Cellular Microbiology
Date: 15 February 2012
Volume: 14
Subjects:
Faculty: Centro de Investigación en Biotecnología y Genómica de Plantas (CBGP) (UPM)
Department: Otro
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

The bacterial pathogen Pseudomonas syringae pv tomato DC3000 suppresses plant innate immunity with effector proteins injected by a type III secretion system (T3SS). The cysteine protease effector HopN1, which reduces the ability of DC3000 to elicit programmed cell death in non-host tobacco, was found to also suppress the production of defence-associated reactive oxygen species (ROS) and callose when delivered by Pseudomonas fluorescens heterologously expressing a P. syringae T3SS. Purified His 6 -tagged HopN1 was used to identify tomato PsbQ, a member of the oxygen evolving complex of photosystem II (PSII), as an interacting protein. HopN1 localized to chloroplasts and both degraded PsbQ and inhibited PSII activity in chloroplast preparations, whereas a HopN1 D299A non-catalytic mutant lost these abilities. Gene silencing of NtPsbQ in tobacco compromised ROS production and programmed cell death.

More information

Item ID: 15600
DC Identifier: http://oa.upm.es/15600/
OAI Identifier: oai:oa.upm.es:15600
DOI: 10.1111/j.1462-5822.2012.01749.x
Deposited by: Memoria Investigacion
Deposited on: 17 Sep 2013 17:21
Last Modified: 21 Apr 2016 15:51
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