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Invariance of the Glass Transition Temperature of Plant Vitrification Solutions with Cooling Rate
Cita
Schneider Teixeira, Aline, Faltus, Milos, Zamecnik, Jiri, Kotková, Renata, González Benito, M. Elena 
ORCID: https://orcid.org/0000-0002-6330-332X and Molina García, Antonio Diego
(2012).
Invariance of the Glass Transition Temperature of Plant Vitrification Solutions with Cooling Rate.
"Cryobiology", v. 65
(n. 3);
p. 347.
ISSN 0011-2240.
ORCID: https://orcid.org/0000-0002-6330-332X and Molina García, Antonio Diego
(2012).
Invariance of the Glass Transition Temperature of Plant Vitrification Solutions with Cooling Rate.
"Cryobiology", v. 65
(n. 3);
p. 347.
ISSN 0011-2240.
Descripción
| Título: | Invariance of the Glass Transition Temperature of Plant Vitrification Solutions with Cooling Rate |
|---|---|
| Autor/es: |
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| Tipo de Documento: | Artículo |
| Título de Revista/Publicación: | Cryobiology |
| Fecha: | Diciembre 2012 |
| ISSN: | 0011-2240 |
| Volumen: | 65 |
| Número: | 3 |
| Materias: | |
| ODS: | |
| Escuela: | E.U.I.T. Agrícolas (UPM) |
| Departamento: | Biología Vegetal [hasta 2014] |
| Licencias Creative Commons: | Reconocimiento - Sin obra derivada - No comercial |
Resumen
The solutions studied were Plant Vitrification Solutions 1, 2 and 3: (PVS1: Uragami et al. 1989, Plant Cell Rep. 8, 418; PVS2: Sakai et al. 1990, Plant Cell Rep. 9, 30; PVS3: Nishizawa et al. 1993, Plant Sci. 91, 67). Cooling was performed using the calorimeter control (5, 10 and 20°C min-1), or for higher rates, by quenching the closed pan with PVS in LN, either naked (faster - 5580°C min-1) or introduced in cryovials (reduced rate 360°C min-1). Quenched pans were then transferred to the sample chamber, pre-cooled to -196°C. Glass transition temperature was observed by DSC with a TA 2920 instrument, upon warming pans with solution samples from -145°C to room temperature, at standard warming rate10°C min-1.
Más información
| ID de Registro: | 16136 |
|---|---|
| Identificador DC: | https://oa.upm.es/16136/ |
| Identificador OAI: | oai:oa.upm.es:16136 |
| Depositado por: | Memoria Investigacion |
| Depositado el: | 17 Jul 2013 09:34 |
| Ultima Modificación: | 03 Jul 2024 09:35 |
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