Invariance of the Glass Transition Temperature of Plant Vitrification Solutions with Cooling Rate

Schneider Teixeira, Aline and Faltus, Milos and Zamecnik, Jiri and Kotková, Renata and González Benito, Elena and Molina García, Antonio Diego (2012). Invariance of the Glass Transition Temperature of Plant Vitrification Solutions with Cooling Rate. "Cryobiology", v. 65 (n. 3); p. 347. ISSN 0011-2240.

Description

Title: Invariance of the Glass Transition Temperature of Plant Vitrification Solutions with Cooling Rate
Author/s:
  • Schneider Teixeira, Aline
  • Faltus, Milos
  • Zamecnik, Jiri
  • Kotková, Renata
  • González Benito, Elena
  • Molina García, Antonio Diego
Item Type: Article
Título de Revista/Publicación: Cryobiology
Date: December 2012
ISSN: 0011-2240
Volume: 65
Subjects:
Faculty: E.U.I.T. Agrícolas (UPM)
Department: Biología Vegetal [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

The solutions studied were Plant Vitrification Solutions 1, 2 and 3: (PVS1: Uragami et al. 1989, Plant Cell Rep. 8, 418; PVS2: Sakai et al. 1990, Plant Cell Rep. 9, 30; PVS3: Nishizawa et al. 1993, Plant Sci. 91, 67). Cooling was performed using the calorimeter control (5, 10 and 20°C min-1), or for higher rates, by quenching the closed pan with PVS in LN, either naked (faster - 5580°C min-1) or introduced in cryovials (reduced rate 360°C min-1). Quenched pans were then transferred to the sample chamber, pre-cooled to -196°C. Glass transition temperature was observed by DSC with a TA 2920 instrument, upon warming pans with solution samples from -145°C to room temperature, at standard warming rate10°C min-1.

More information

Item ID: 16136
DC Identifier: http://oa.upm.es/16136/
OAI Identifier: oai:oa.upm.es:16136
Deposited by: Memoria Investigacion
Deposited on: 17 Jul 2013 09:34
Last Modified: 21 Apr 2016 16:28
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