Palacín Gómez, Aranzazu and Gomez Casado, Cristina and Rivas, Luis and Aguirre, Jacobo and Tordesillas Villuendas, Leticia and Bartra, Joan and Blanco, Carlos and Carrillo, Teresa and Cuesta-Herranz, Javier and Frutos, Consolación de and García Álvarez-Eire, Genoveva and Fernández, Francisco and Gamboa, P.M. and Muñoz, Rosa and Sánchez-Monge Laguna de Rins, Rosa and Sirvent, Sofía and Torres, María and Varela Losada, Susana and Rodríguez, Rosalía and Parro, Victor and Blanca, Miguel and Salcedo Duran, Gabriel and Díaz Perales, Araceli
Graph based study of allergen cross-reactivity of plant lipid transfer proteins (LTPs) using microarray in a multicenter study..
"Plos One", v. 7
(n. 12 e50);
The study of cross-reactivity in allergy is key to both understanding. the allergic response of many patients and providing them with a rational treatment In the present study, protein microarrays and a co-sensitization graph approach were used in conjunction with an allergen microarray immunoassay. This enabled us to include a wide number of proteins and a large number of patients, and to study sensitization profiles among members of the LTP family. Fourteen LTPs from the most frequent plant food-induced allergies in the geographical area studied were printed into a microarray specifically designed for this research. 212 patients with fruit allergy and 117 food-tolerant pollen allergic subjects were recruited from seven regions of Spain with different pollen profiles, and their sera were tested with allergen microarray. This approach has proven itself to be a good tool to study cross-reactivity between members of LTP family, and could become a useful strategy to analyze other families of allergens.