Fusarium proliferatum isolated from garlic in Spain: Identification, toxigenic potential and pathogenicity on related Allium species

Palmero Llamas, Daniel and Cara Garcia, Miguel de and Nosir, W. and Galvez Patón, Laura and Cruz, Alejandra and Woodward, S. and González Jaén, María Teresa and Tello Marquina, Julio (2012). Fusarium proliferatum isolated from garlic in Spain: Identification, toxigenic potential and pathogenicity on related Allium species. "Phytopathologia Mediterranea", v. 51 (n. 1); pp. 207-218. ISSN 0031-9465.

Description

Title: Fusarium proliferatum isolated from garlic in Spain: Identification, toxigenic potential and pathogenicity on related Allium species
Author/s:
  • Palmero Llamas, Daniel
  • Cara Garcia, Miguel de
  • Nosir, W.
  • Galvez Patón, Laura
  • Cruz, Alejandra
  • Woodward, S.
  • González Jaén, María Teresa
  • Tello Marquina, Julio
Item Type: Article
Título de Revista/Publicación: Phytopathologia Mediterranea
Date: 2012
Volume: 51
Subjects:
Faculty: E.U.I.T. Agrícolas (UPM)
Department: Producción Vegetal: Botánica y Protección Vegetal [hasta 2014]
Creative Commons Licenses: Recognition - No derivative works - Non commercial

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Abstract

Fusarium proliferatum has been reported on garlic in the Northwest USA, Spain and Serbia, causing water-soaked tan-colored lesions on cloves. In this work, Fusarium proliferatum was isolated from 300 symptomatic garlic bulbs. Morphological identification of Fusarium was confirmed using species-specific PCR assays and EF-1α sequencing. Confirmation of pathogenicity was conducted with eighteen isolates. Six randomly selected F. proliferatum isolates from garlic were tested for specific pathogenicity and screened for fusaric acid production. Additionally, pathogenicity of each F. proliferatum isolate was tested on healthy seedlings of onion (Allium cepa), leek (A. porrum), scallions (A. fistulosum), chives (A. schoenoprasum) and garlic (A. sativum). A disease severity index (DSI) was calculated as the mean severity on three plants of each species with four test replicates. Symptoms on onion and garlic plants were observed three weeks after inoculation. All isolates tested produced symptoms on all varieties inoculated. Inoculation of F. proliferatum isolates from diseased garlic onto other Allium species provided new information on host range and pathogenicity. The results demonstrated differences in susceptibility with respect to host species and cultivar. The F. proliferatum isolates tested all produced fusaric acid (FA); correlations between FA production and isolate pathogenicity are discussed. Additionally, all isolates showed the presence of the FUM1 gene suggesting the ability of Spanish isolates to produce fumonisins.

More information

Item ID: 16605
DC Identifier: http://oa.upm.es/16605/
OAI Identifier: oai:oa.upm.es:16605
Deposited by: Memoria Investigacion
Deposited on: 14 Oct 2013 09:14
Last Modified: 21 Apr 2016 16:55
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